Rabbit anti-Human WNT3 Polyclonal Antibody | anti-WNT3 antibody

WNT3, ID (WNT3, INT4, Proto-oncogene Wnt-3, Proto-oncogene Int-4 homolog) (Biotin)

Cat. Num. AAA6363700

• Gene Names: WNT3; INT4; TETAMS
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: WNT3; Polyclonal Antibody; ID (WNT3; INT4; Proto-oncogene Wnt-3; Proto-oncogene Int-4 homolog) (Biotin); Proto-oncogene Int-4 homolog; anti-WNT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human WNT3.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-WNT3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 155-184 in the central region of human WNT3, UniProt Accession #P56703; NP_110380.1. Species sequence homology: chicken, mouse, xenopus
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of CEM cell line lysates (35ug/lane) usingMBS6012626. This demonstrates MBS6012626 detected the WNT3 protein (arrow).)

Related Product Information for anti-WNT3 antibody

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region.

Product Categories/Family for anti-WNT3 antibody

Antibodies; Wnt Proteins

References

Nikopensius, T., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):748-756(2010). Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010). Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010). Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010). Memarian, A., et al. Leuk. Lymphoma 50(12):2061-2070(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 13540477
• NCBI GeneID: 7473
• NCBI Accession #: NP_110380.1
• NCBI GenBank Nucleotide #: NM_030753.4
• UniProt Accession #: P56703; Q2M237; Q9H1J9
• Molecular Weight: 39,645 Da
• NCBI Official Full Name: proto-oncogene Wnt-3
• NCBI Official Synonym Full Names: Wnt family member 3
• NCBI Official Symbol: WNT3
• NCBI Official Synonym Symbols: INT4; TETAMS
• NCBI Protein Information: proto-oncogene Wnt-3
• UniProt Protein Name: Proto-oncogene Wnt-3
• Protein Family: Proto-oncogene
• UniProt Gene Name: WNT3
• UniProt Synonym Gene Names: INT4
• UniProt Entry Name: WNT3_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT3: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Defects in WNT3 are the cause of autosomal recessive tetra-amelia (ARTTRA). Tetra-amelia is a rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects. Belongs to the Wnt family.

Protein type: Secreted; Oncoprotein; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extracellular space; proteinaceous extracellular matrix; Golgi lumen; endoplasmic reticulum lumen; extracellular region; plasma membrane

Molecular Function: protein domain specific binding; protein binding; frizzled binding; receptor agonist activity

Biological Process: embryonic forelimb morphogenesis; axon guidance; cell fate commitment; cell morphogenesis; dorsal/ventral axis specification; Wnt receptor signaling pathway through beta-catenin; positive regulation of collateral sprouting in the absence of injury; negative regulation of axon extension involved in axon guidance; embryonic hindlimb morphogenesis; Spemann organizer formation at the anterior end of the primitive streak; neuron differentiation; limb bud formation; mesoderm formation; gamete generation; anterior/posterior axis specification

Disease: Tetraamelia, Autosomal Recessive