Rabbit WNK1 Polyclonal Antibody | anti-WNK1 antibody

WNK1 (Phospho-T60) Polyclonal Antibody

Cat. Num. AAA3004848

• Gene Names: WNK1; KDP; PSK; p65; HSN2; HSAN2; PRKWNK1; PPP1R167
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: WNK1; Polyclonal Antibody; WNK1 (Phospho-T60) Polyclonal Antibody; Serine/threonine-protein kinase WNK1; Erythrocyte 65 kDa protein; Kinase deficient protein; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; hWNK1; WNK; HSN2; KDP; KIAA0344; PRKWNK1; anti-WNK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-WNK1 (T60) polyclonal antibody detects endogenous levels of WNK1 protein only when phosphorylated at Thr60.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 2642

• Applicable Applications for anti-WNK1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human WNK1 around the phosphorylation site of Threonine 60.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-WNK1 (T60) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T treated with EGF (100ng/ml,30 minutes) whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-WNK1 (T60) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-WNK1 antibody

WNK1 (WNK lysine deficient protein kinase 1), also known as KDP (kinase deficient protein), PSK, erythrocyte 65 kDa protein (p65), HSN2, HSAN2 or PRKWNK1, is a 2, 382 amino acid cytoplasmic protein that participates in cell signaling, proliferation and survival, and regulates electrolyte homeostasis. WNK1 belongs to the Ser/Thr protein kinase family of the protein kinase superfamily, and contains one protein kinase domain. Existing as five alternatively spliced isoforms, WNK1 is widely expressed but is found at highest levels in skeletal muscke, heart, testis and kidney. The gene that encodes WNK1 maps to human chromosome 12p13.33, and when defective, is the cause of an an autosomal dominant disease known as pseudohypoaldosteronism type II (PHAII), as well as a hereditary sensory and autonomic neuropathy designated hereditary sensory and autonomic neuropathy type 2A (HSAN2A).

NCBI and Uniprot Product Information

• NCBI GI #: 296939600
• NCBI GeneID: 65125
• NCBI Accession #: NP_001171914.1
• NCBI GenBank Nucleotide #: NP_001171914.1
• UniProt Accession #: Q9H4A3
• Molecular Weight: ~ 251kDa
• NCBI Official Full Name: serine/threonine-protein kinase WNK1 isoform 4
• NCBI Official Synonym Full Names: WNK lysine deficient protein kinase 1
• NCBI Official Symbol: WNK1
• NCBI Official Synonym Symbols: KDP; PSK; p65; HSN2; HSAN2; PRKWNK1; PPP1R167
• NCBI Protein Information: serine/threonine-protein kinase WNK1
• UniProt Protein Name: Serine/threonine-protein kinase WNK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: WNK1
• UniProt Synonym Gene Names: HSN2; KDP; KIAA0344; PRKWNK1; p65; hWNK1
• UniProt Entry Name: WNK1_HUMAN

NCBI Description

This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]

Uniprot Description

WNK1: a serine-threonine protein kinase that controls sodium and chloride ion transport. May regulate the activity of the thiazide-sensitive Na-Cl cotransporter SLC12A3 by phosphorylation. May also play a role in actin cytoskeletal reorganization.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, Other; Other group; Wnk family

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; chloride channel inhibitor activity; protein kinase inhibitor activity; ATP binding; phosphatase binding

Biological Process: regulation of cellular process; peptidyl-threonine phosphorylation; negative regulation of protein kinase activity; neuron development; ion transport; protein amino acid phosphorylation

Disease: Pseudohypoaldosteronism, Type Iic; Neuropathy, Hereditary Sensory And Autonomic, Type Iia