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Western Blot (WB) (WIPF2 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the WIPF2 antibody detected the WIPF2 protein (arrow).)

Rabbit anti-Human WIPF2 Polyclonal Antibody | anti-WIPF2 antibody

WIPF2, CT (WIPF2, WICH, WIRE, WAS/WASL-interacting protein family member 2, WASP-interacting protein-related protein, WIP-and CR16-homologous protein, WIP-related protein) (Biotin)

Gene Names
WIPF2; WICH; WIRE
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
WIPF2; Polyclonal Antibody; CT (WIPF2; WICH; WIRE; WAS/WASL-interacting protein family member 2; WASP-interacting protein-related protein; WIP-and CR16-homologous protein; WIP-related protein) (Biotin); WIP-related protein; anti-WIPF2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-WIPF2 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
WIPF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 392-419 amino acids from the C-terminal region of human WIPF2.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WIPF2 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the WIPF2 antibody detected the WIPF2 protein (arrow).)

Western Blot (WB) (WIPF2 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the WIPF2 antibody detected the WIPF2 protein (arrow).)

Immunohistochemistry (IHC)

(WIPF2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WIPF2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (WIPF2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WIPF2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-WIPF2 antibody
This gene encodes a WASP interacting protein (WIP)-related protein. It has been shown that this protein has a role in the WASP-mediated organization of the actin cytoskeleton and that this protein is a potential link between the activated platelet-derived growth factor receptor and the actin polymerization machinery.
Product Categories/Family for anti-WIPF2 antibody
References
Wan, D., et al. Proc. Natl. Acad. Sci. U.S.A. 101(44):15724-15729(2004). Salazar, M.A., et al. J. Biol. Chem. 278(49):49031-49043(2003). Aspenstrom, P. Exp. Cell Res. 279(1):21-33(2002). Kato, M., et al. Biochem. Biophys. Res. Commun. 291(1):41-47(2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,289 Da
NCBI Official Full Name
WAS/WASL-interacting protein family member 2
NCBI Official Synonym Full Names
WAS/WASL interacting protein family, member 2
NCBI Official Symbol
WIPF2
NCBI Official Synonym Symbols
WICH; WIRE
NCBI Protein Information
WAS/WASL-interacting protein family member 2; WIP-related protein; WASP-binding protein; WIP- and CR16-homologous protein; WASP-interacting protein-related protein
UniProt Protein Name
WAS/WASL-interacting protein family member 2
UniProt Gene Name
WIPF2
UniProt Synonym Gene Names
WICH; WIRE
UniProt Entry Name
WIPF2_HUMAN

NCBI Description

This gene encodes a WASP interacting protein (WIP)-related protein. It has been shown that this protein has a role in the WASP-mediated organization of the actin cytoskeleton and that this protein is a potential link between the activated platelet-derived growth factor receptor and the actin polymerization machinery. [provided by RefSeq, Jul 2008]

Uniprot Description

WIRE: Plays an active role in the formation of cell surface protrusions downstream of activated PDGFB receptors. Plays an important role in actin-microspike formation through cooperation with WASL. May cooperate with WASP and WASL to induce mobilization and reorganization of the actin filament system. Belongs to the verprolin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoskeleton; cytosol

Molecular Function: protein binding; actin binding

Biological Process: innate immune response

Research Articles on WIPF2

Similar Products

Product Notes

The WIPF2 wipf2 (Catalog #AAA6363623) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WIPF2, CT (WIPF2, WICH, WIRE, WAS/WASL-interacting protein family member 2, WASP-interacting protein-related protein, WIP-and CR16-homologous protein, WIP-related protein) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WIPF2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the WIPF2 wipf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WIPF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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