Rabbit wg Polyclonal Antibody | anti-wg antibody
Rabbit anti-Drosophila melanogaster (Fruit fly) wg Polyclonal Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
2. 1mL preimmune serum (negative control)
3. 10mg rabbit polyclonal antibody purified by Protein A/G.
2. Antibody titer > 1: 64,000 confirmed by ELISA
3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
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NCBI and Uniprot Product Information
Uniprot Description
Function: Binds as a ligand to a family of frizzled seven-transmembrane receptors and acts through a cascade of genes on the nucleus. Segment polarity protein. May be a growth factor. Acts on neighboring cells to regulate at least one gene, the homeobox segmentation gene engrailed. Wg signal represses arm phosphorylation. Wg signaling operates by inactivating the sgg repression of engrailed autoactivation. Wg and Wnt2 have a role in the developing trachea and together are responsible for all dorsal trunk formation. Wg also acts in the developing epidermis. Acts as a morphogen, and diffuses long distances despite its lipidation. Lipophorin is required for diffusion, probably by acting as vehicle for its movement, explaining how it can spread over long distances despite its lipidation. In non-neuronal cells, wls directs wg secretion via clathrin-mediated endocytosis and the retromer complex (a conserved protein complex consisting of Vps26 and Vps35) to sustain a wls traffic loop encompassing the Golgi, the cell surface, an endocytic compartment and a retrograde route leading back to the Golgi. In neuronal cells (the larval motorneuron NMJ), wg signal moves across the synapse through the release of wls-containing exosome-like vesicles. Ref.11 Ref.15 Ref.16 Ref.17
Subunit structure: Monomer; folds by intramolecular disulfide bonds. Interacts with porcupine (por). Interacts with wls; in the Golgi. Ref.8 Ref.12 Ref.15
Subcellular location: Secreted. Cell junction › synapse. Membrane; Lipid-anchor. Secreted › extracellular space › extracellular matrix. Note: Palmitoylation converts wg into a membrane-anchored protein that is partitioned into specialized lipid raft microdomains before secretion. Possibly associated with the extracellular matrix. Ref.3 Ref.13 Ref.15 Ref.17
Tissue specificity: Segmented expression in embryos. In embryonic tracheal cells, expression is in stripes flanking the tracheal placode. Ref.1 Ref.11
Developmental stage: Expressed throughout development, but barely detectable in adults. Ref.1 Ref.2
Post-translational modification: Palmitoylated by porcupine, acylation is a prerequisite. The lipid group functions as a sorting signal, targeting the ligand to polarized vesicles that transport wg to unique sites at the cell surface. Major form is glycosylated at 2 sites, glycosylation is stimulated by porcupine at the ER, again acylation is a prerequisite. Ref.12
Disruption phenotype: Accumulation in the Golgi, thereby preventing secretion. Ref.16
Sequence similarities: Belongs to the Wnt family.
Research Articles on wg
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Product Notes
The wg wg (Catalog #AAA7164863) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Drosophila melanogaster (Fruit fly) wg Polyclonal Antibody reacts with Drosophila melanogaster (Fruit fly) and may cross-react with other species as described in the data sheet. AAA Biotech's wg can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), WB (Western Blot) (ensure identification of antigen). Researchers should empirically determine the suitability of the wg wg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "wg, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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