Rabbit anti-Human WFDC1 Polyclonal Antibody | anti-WFDC1 antibody

WFDC1, CT (H163) (WFDC1, PS20, WAP four-disulfide core domain protein 1, Prostate stromal protein ps20, ps20 growth inhibitor) (FITC)

Cat. Num. AAA6363536

• Gene Names: WFDC1; PS20
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: WFDC1; Polyclonal Antibody; CT (H163) (WFDC1; PS20; WAP four-disulfide core domain protein 1; Prostate stromal protein ps20; ps20 growth inhibitor) (FITC); ps20 growth inhibitor; anti-WFDC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-WFDC1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: WFDC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-177 amino acids from the C-terminal region of human WFDC1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WFDC1 Antibody (C-term H163) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the WFDC1 antibody detected the WFDC1 protein (arrow).)

Immunohistochemistry (IHC)

(WFDC1 Antibody (C-term H163) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WFDC1 Antibody (C-term H163) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(WFDC1 Antibody (C-term H163) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-WFDC1 antibody

This gene encodes a member of the WAP-type four disulfide core domain family. The WAP-type four-disulfide core domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. The encoded protein shares 81% amino acid identity with the rat ps20 protein, which was originally identified as a secreted growth inhibitor. This gene is mapped to chromosome 16q24, an area of frequent loss of heterozygosity in cancers, including prostate, breast and hepatocellular cancers and Wilms' tumor. Owing to its location and a possible growth inhibitory property of its gene product, this gene is suggested to be a tumor suppressor gene.

Product Categories/Family for anti-WFDC1 antibody

Antibodies; Disease Markers

References

Briggs, F.B., et al. Genes Immun. 11(3):199-208(2010). Madar, S., et al. Carcinogenesis 30(1):20-27(2009). Liu, S., et al. Clin. Exp. Metastasis 26(7):739-749(2009). Alvarez, R., et al. J. Virol. 82(1):471-486(2008). Watson, J.E., et al. Prostate 61(2):192-199(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 18379353
• NCBI GeneID: 58189
• NCBI Accession #: NP_067020.2
• NCBI GenBank Nucleotide #: NM_021197.3
• UniProt Accession #: Q9HC57; Q8NC27; Q9HAU1; D3DUL7
• Molecular Weight: 23,977 Da
• NCBI Official Full Name: WAP four-disulfide core domain protein 1 isoform 1
• NCBI Official Synonym Full Names: WAP four-disulfide core domain 1
• NCBI Official Symbol: WFDC1
• NCBI Official Synonym Symbols: PS20
• NCBI Protein Information: WAP four-disulfide core domain protein 1
• UniProt Protein Name: WAP four-disulfide core domain protein 1
• Protein Family: WAP four-disulfide core domain protein
• UniProt Gene Name: WFDC1
• UniProt Synonym Gene Names: PS20
• UniProt Entry Name: WFDC1_HUMAN

NCBI Description

This gene encodes a member of the WAP-type four disulfide core domain family. The WAP-type four-disulfide core domain contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is mapped to chromosome 16q24, an area of frequent loss of heterozygosity in cancers, including prostate, breast and hepatocellular cancers and Wilms' tumor. This gene is downregulated in many cancer types and may be involved in the inhibition of cell proliferation. The encoded protein may also play a role in the susceptibility of certain CD4 memory T cells to human immunodeficiency virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

WFDC1: Has growth inhibitory activity.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: negative regulation of cell growth; negative regulation of epithelial cell proliferation; negative regulation of inflammatory response; regulation of cell growth; response to drug; response to estradiol stimulus