Western Blot (WB)
(Western blot analysis of IL12RB2 expression in 721_B whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse IL12RB2 Polyclonal Antibody | anti-IL12RB2 antibody
IL12RB2 Antibody
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
IL12RB2; Polyclonal Antibody; IL12RB2 Antibody; I12R2_HUMAN; IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12R-beta-2; IL-12RB2; IL12 receptor beta 2; IL12R beta2; Interleukin 12 receptor beta 2; Interleukin 12 receptor beta 2 chain; Interleukin-12 receptor subunit beta-2; RP11 102M16.1; anti-IL12RB2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
IL12RB2 antibody detects endogenous levels of total IL12RB2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
659
Applicable Applications for anti-IL12RB2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide
Subcellular Location
Membrane.
Tissue Specificity
Isoform 2 is expressed at similar levels in both naive and activated T-cells.
Predicted Cross Reactivity
Pig, Bovine, Dog
Similarity
Pig (83%), Bovine (92%), Dog (83%)
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of IL12RB2 expression in 721_B whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of IL12RB2 expression in 721_B whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-IL12RB2 antibody
Description: Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.
Subunit Structure: Heterodimer/heterooligomer; disulfide-linked. The functional high affinity IL12 receptor is composed of I12RB1 and IL12RB2. Il12RB2 binds JAK2 (via its N-terminal) through a membrane-proximal region of the cytoplasmic domain. Interaction, in vitro and in vivo, with SOCS3 (via its SH2 domain) inhibits the STAT4-mediated activation. Binds STAT4 through a membrane-distal C-terminal region.
Post-translational Modifications: On IL12 binding, phosphorylated on C-terminal tyrosine residues by JAK2. Phosphorylation on Tyr-800 is required for STAT4 binding and activation, and for SOCS3 binding.
Similarity: The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.The box 1 motif is required for JAK interaction and/or activation. Belongs to the type I cytokine receptor family. Type 2 subfamily.
Subunit Structure: Heterodimer/heterooligomer; disulfide-linked. The functional high affinity IL12 receptor is composed of I12RB1 and IL12RB2. Il12RB2 binds JAK2 (via its N-terminal) through a membrane-proximal region of the cytoplasmic domain. Interaction, in vitro and in vivo, with SOCS3 (via its SH2 domain) inhibits the STAT4-mediated activation. Binds STAT4 through a membrane-distal C-terminal region.
Post-translational Modifications: On IL12 binding, phosphorylated on C-terminal tyrosine residues by JAK2. Phosphorylation on Tyr-800 is required for STAT4 binding and activation, and for SOCS3 binding.
Similarity: The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.The box 1 motif is required for JAK interaction and/or activation. Belongs to the type I cytokine receptor family. Type 2 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 75 kDa
Predicted: 98 kDa
Predicted: 98 kDa
NCBI Official Full Name
interleukin-12 receptor subunit beta-2 isoform b
NCBI Official Synonym Full Names
interleukin 12 receptor subunit beta 2
NCBI Official Symbol
IL12RB2
NCBI Protein Information
interleukin-12 receptor subunit beta-2
UniProt Protein Name
Interleukin-12 receptor subunit beta-2
Protein Family
UniProt Gene Name
IL12RB2
UniProt Synonym Gene Names
IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12R-beta-2; IL-12RB2
NCBI Description
The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. Several transcript variants encoding different isoforms and non-protein coding transcripts have been found for this gene. [provided by RefSeq, Apr 2012]
Uniprot Description
Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.
Research Articles on IL12RB2
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Product Notes
The IL12RB2 il12rb2 (Catalog #AAA9604023) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL12RB2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL12RB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the IL12RB2 il12rb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL12RB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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