Western Blot (WB)
(Western blot analysis of lysates from HUVEC cells, using WEE2 Antibody. The lane on the right is blocked with the synthesized peptide.)
Rabbit anti-Human WEE2 Polyclonal Antibody | anti-WEE2 antibody
WEE2 Antibody
Gene Names
WEE2; WEE1B
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
WEE2; Polyclonal Antibody; WEE2 Antibody; anti-WEE2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
WEE2 Antibody detects endogenous levels of total WEE2 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
567
Applicable Applications for anti-WEE2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:5000
ELISA: 1:5000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human WEE2.
Immunogen Range
151-200
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Western Blot (WB)
(Western blot analysis of lysates from HUVEC cells, using WEE2 Antibody. The lane on the right is blocked with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of lysates from HUVEC cells, using WEE2 Antibody. The lane on the right is blocked with the synthesized peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
62 kDa
NCBI Official Full Name
wee1-like protein kinase 2
NCBI Official Synonym Full Names
WEE1 homolog 2
NCBI Official Symbol
WEE2
NCBI Official Synonym Symbols
WEE1B
NCBI Protein Information
wee1-like protein kinase 2
UniProt Protein Name
Wee1-like protein kinase 2
Protein Family
UniProt Gene Name
WEE2
UniProt Synonym Gene Names
WEE1B
Uniprot Description
Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation ().
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Product Notes
The WEE2 wee2 (Catalog #AAA9507093) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WEE2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WEE2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:5000. Researchers should empirically determine the suitability of the WEE2 wee2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WEE2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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