Mouse ID ELISA Kit | ID elisa kit

Mouse ID(deiodinase) ELISA Kit

Cat. Num. AAA2512756

• Gene Names: ID1; ID; bHLHb24
• Reactivity: Mouse
• Synonyms: ID; Mouse ID(deiodinase) ELISA Kit; ID elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant Mouse ID. No significant cross-reactivity or interference between Mouse ID and analogues was observed.
• Sequence Length: 155

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ID elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse ID concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ID. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ID and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ID, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ID. You can calculate the concentration of ID in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15214589
• NCBI GeneID: 3397
• UniProt Accession #: P41134; O00651; O00652; Q16371; Q16377; Q5TE66; Q5TE67; Q969Z7; Q9H0Z5; Q9H109; A8K537; E1P5L4
• Molecular Weight: 15,586 Da
• NCBI Official Full Name: Inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
• NCBI Official Synonym Full Names: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
• NCBI Official Symbol: ID1
• NCBI Official Synonym Symbols: ID; bHLHb24
• NCBI Protein Information: DNA-binding protein inhibitor ID-1; class B basic helix-loop-helix protein 24; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein); inhibitor of differentiation 1
• UniProt Protein Name: DNA-binding protein inhibitor ID-1
• Protein Family: IDLSRF-like peptide
• UniProt Gene Name: ID1
• UniProt Synonym Gene Names: BHLHB24; ID; bHLHb24
• UniProt Entry Name: ID1_HUMAN

NCBI Description

The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ID1: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 20q11

Cellular Component: nucleoplasm; Golgi apparatus; nucleus

Molecular Function: protein dimerization activity; protein binding; transcription factor activity

Biological Process: circadian rhythm; transcription, DNA-dependent; blood vessel endothelial cell migration; protein destabilization; collagen metabolic process; heart development; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; regulation of angiogenesis; negative regulation of DNA binding; response to antibiotic; BMP signaling pathway; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; blood vessel morphogenesis; negative regulation of osteoblast differentiation; endothelial cell morphogenesis; angiogenesis; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis