Western Blot (WB)
(Mouse Wee1 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the Wee1 antibody detected the Wee1 protein (arrow).)
Rabbit Wee1 Polyclonal Antibody | anti-Wee1 antibody
Mouse Wee1 Antibody (Center)
Gene Names
Wee1; Wee1A
Reactivity
Human (Predicted Reactivity: Rat)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Wee1; Polyclonal Antibody; Mouse Wee1 Antibody (Center); Wee1-like protein kinase; Wee1A kinase; anti-Wee1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Wee1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-230 amino acids from the Central region of mouse Wee1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.4 (varies by lot)
Sequence Positions
202-230
Sequence Length
646
Applicable Applications for anti-Wee1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Mouse Wee1 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the Wee1 antibody detected the Wee1 protein (arrow).)
Western Blot (WB)
(Mouse Wee1 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the Wee1 antibody detected the Wee1 protein (arrow).)
Related Product Information for anti-Wee1 antibody
Wee1 may act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur (By similarity).
Product Categories/Family for anti-Wee1 antibody
References
Muller, M., et al. J. Cell. Sci. 123 (PT 2), 286-294 (2010) :
Kim, M.J., et al. Oncol. Rep. 19(5):1323-1329(2008)
Tanaka, Y., et al. Biochem. Biophys. Res. Commun. 352(1):21-28(2007)
Tominaga, Y., et al. Int. J. Biol. Sci. 2(4):161-170(2006)
Park, C.E., et al. Cells Tissues Organs (Print) 177(4):221-228(2004)
Kim, M.J., et al. Oncol. Rep. 19(5):1323-1329(2008)
Tanaka, Y., et al. Biochem. Biophys. Res. Commun. 352(1):21-28(2007)
Tominaga, Y., et al. Int. J. Biol. Sci. 2(4):161-170(2006)
Park, C.E., et al. Cells Tissues Organs (Print) 177(4):221-228(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71578
NCBI Official Full Name
wee1-like protein kinase
NCBI Official Synonym Full Names
WEE 1 homolog 1 (S. pombe)
NCBI Official Symbol
Wee1
NCBI Official Synonym Symbols
Wee1A
NCBI Protein Information
wee1-like protein kinase
UniProt Protein Name
Wee1-like protein kinase
Protein Family
UniProt Gene Name
Wee1
UniProt Entry Name
WEE1_MOUSE
Uniprot Description
Wee1: a protein kinase of the WEE family. A nuclear protein which catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
Protein type: Kinase, protein; EC 2.7.10.2; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Other group; WEE family
Cellular Component: cytoplasm; nucleus
Molecular Function: transferase activity; protein binding; protein-tyrosine kinase activity; metal ion binding; transferase activity, transferring phosphorus-containing groups; magnesium ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; kinase activity; ATP binding; protein kinase activity
Biological Process: mitosis; peptidyl-tyrosine phosphorylation; cell division; establishment of cell polarity; microtubule cytoskeleton organization and biogenesis; cell cycle; phosphorylation; protein amino acid phosphorylation; neurite morphogenesis
Research Articles on Wee1
Similar Products
Product Notes
The Wee1 wee1 (Catalog #AAA9208291) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 202-230. The Mouse Wee1 Antibody (Center) reacts with Human (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Wee1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Wee1 wee1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wee1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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