Rabbit anti-Human Wee1 Polyclonal Antibody | anti-Wee1 antibody

Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase) (MaxLight 550)

Cat. Num. AAA6363518

• Gene Names: Wee1; Wee1A
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Wee1; Polyclonal Antibody; ID (Wee1; Wee1-like protein kinase; Wee1A kinase) (MaxLight 550); Wee1A kinase; anti-Wee1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Wee1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-Wee1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 202-230 in the central terminal region of mouse Wee1. Species sequence homology: rat
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in WiDr cell line lysates (35ug/lane) usingMBS6004282. This demonstrates that MBS6004282 detected the Wee1 protein (arrow).)

Related Product Information for anti-Wee1 antibody

Wee1 may act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur (By similarity).

Product Categories/Family for anti-Wee1 antibody

Antibodies; Protein Kinases

References

1. Muller, M., et al. J. Cell. Sci. 123 (PT 2), 286-294 (2010). 2. Kim, M.J., et al. Oncol. Rep. 19(5):1323-1329(2008). 3. Tanaka, Y., et al. Biochem. Biophys. Res. Commun. 352(1):21-28(2007). 4. Tominaga, Y., et al. Int. J. Biol. Sci. 2(4):161-170(2006). 5. Park, C.E., et al. Cells Tissues Organs (Print) 177(4):221-228(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 31543956
• NCBI GeneID: 22390
• NCBI Accession #: NP_033542.2
• NCBI GenBank Nucleotide #: NM_009516.3
• UniProt Accession #: P47810; Q9EPL7
• Molecular Weight: 71,578 Da
• NCBI Official Full Name: wee1-like protein kinase isoform 1
• NCBI Official Synonym Full Names: WEE 1 homolog 1 (S. pombe)
• NCBI Official Symbol: Wee1
• NCBI Official Synonym Symbols: Wee1A
• NCBI Protein Information: wee1-like protein kinase
• UniProt Protein Name: Wee1-like protein kinase
• Protein Family: Wee1-like protein kinase
• UniProt Gene Name: Wee1
• UniProt Entry Name: WEE1_MOUSE

Uniprot Description

Wee1: a protein kinase of the WEE family. A nuclear protein which catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Protein type: EC 2.7.10.2; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; WEE family

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function: ATP binding; kinase activity; magnesium ion binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein kinase activity; protein-tyrosine kinase activity; transferase activity

Biological Process: cell cycle; cell division; establishment of cell polarity; microtubule cytoskeleton organization and biogenesis; mitosis; neurite morphogenesis; peptidyl-tyrosine phosphorylation; phosphorylation; protein amino acid phosphorylation