Rabbit anti-Human, Mouse WDYHV1 Polyclonal Antibody | anti-WDYHV1 antibody

WDYHV1, ID (Protein N-terminal Glutamine Amidohydrolase, Protein NH2-Terminal Glutamine Deamidase, N-terminal Gln Amidase, Nt(Q)-amidase, WDYHV Motif-containing Protein 1, NTAQ1, C8orf32)

Cat. Num. AAA630573

• Gene Names: WDYHV1; C8orf32; MGC127299; C14H8orf32
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: WDYHV1; Polyclonal Antibody; ID (Protein N-terminal Glutamine Amidohydrolase; Protein NH2-Terminal Glutamine Deamidase; N-terminal Gln Amidase; Nt(Q)-amidase; WDYHV Motif-containing Protein 1; NTAQ1; C8orf32); Anti -WDYHV1; anti-WDYHV1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human WDYHV1. Species Crossreactivity: mouse.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-WDYHV1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:1000; Western Blot: 1:50-1:100; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide corresponding to aa114-143 from from the center region of human WDYHV1, conjugated to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-WDYHV1 Antibody (Center) Pab in mouse heart tissue lysates (35ug/lane). WDYHV1(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with C8orf32 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-WDYHV1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 74353980
• NCBI GeneID: 510463
• UniProt Accession #: Q3T0D3
• Molecular Weight: 23,910 Da
• NCBI Official Full Name: WDYHV motif containing 1
• NCBI Official Symbol: WDYHV1
• NCBI Official Synonym Symbols: C8orf32; MGC127299; C14H8orf32
• NCBI Protein Information: protein N-terminal glutamine amidohydrolase; nt(Q)-amidase; N-terminal Gln amidase; WDYHV motif-containing protein 1; protein NH2-terminal glutamine deamidase
• UniProt Protein Name: Protein N-terminal glutamine amidohydrolase
• UniProt Gene Name: WDYHV1
• UniProt Synonym Gene Names: NTAQ1
• UniProt Entry Name: NTAQ1_BOVIN

Uniprot Description

Function: Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine

Subunit structure: Monomer

Subcellular location: Cytoplasm › cytosol

By similarity. Nucleus

Sequence similarities: Belongs to the NTAQ1 family.