Western Blot (WB)
(Western blot analysis of extracts from COLO cells, using MOL2B antibody.)
Rabbit anti-Human, Mouse MOBKL2B Polyclonal Antibody | anti-MOBKL2B antibody
MOBKL2B Antibody
Gene Names
MOB3B; MOB1D; C9orf35; MOBKL2B
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MOBKL2B; Polyclonal Antibody; MOBKL2B Antibody; Mob1 homolog 2b; MOB1 Mps One Binder kinase activator like 2B (yeast); MOB1 Mps One Binder kinase activator like 2B; MOB3B; MOL2B_HUMAN; monopolar spindle 1 binding MOB1 domain containing; Mps one binder kinase activator-like 2B; Protein Mob3B; anti-MOBKL2B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
MOBKL2B antibody detects endogenous levels of total MOBKL2B
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
216
Applicable Applications for anti-MOBKL2B antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from COLO cells, using MOL2B antibody.)
Western Blot (WB)
(Western blot analysis of extracts from COLO cells, using MOL2B antibody.)
Immunofluorescene (IF)
(MBS9604798 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9604798 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-MOBKL2B antibody
Function: May regulate the activity of kinases.
Similarity: Belongs to the MOB1/phocein family.
Similarity: Belongs to the MOB1/phocein family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 23 kD
Predicted: 26 kDa
Predicted: 26 kDa
NCBI Official Full Name
MOB kinase activator 3B
NCBI Official Synonym Full Names
MOB kinase activator 3B
NCBI Official Symbol
MOB3B
NCBI Official Synonym Symbols
MOB1D; C9orf35; MOBKL2B
NCBI Protein Information
MOB kinase activator 3B
UniProt Protein Name
MOB kinase activator 3B
UniProt Gene Name
MOB3B
UniProt Synonym Gene Names
MOB kinase activator-like 2B
NCBI Description
The protein encoded by this gene shares similarity with the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. This gene is located on the opposite strand as the interferon kappa precursor (IFNK) gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Modulates LATS1 expression in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis.
Research Articles on MOBKL2B
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Product Notes
The MOBKL2B mob3b (Catalog #AAA9604798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MOBKL2B Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MOBKL2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the MOBKL2B mob3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MOBKL2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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