Rabbit anti-Human, Mouse WDYHV1 Polyclonal Antibody | anti-WDYHV1 antibody

WDYHV1 Antibody

Cat. Num. AAA1497844

• Gene Names: WDYHV1; C8orf32
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: >95%, Protein G Purified
• Synonyms: WDYHV1; Polyclonal Antibody; WDYHV1 Antibody; Protein N-terminal glutamine amidohydrolase (EC:3.5.1.-); C8orf32; NTAQ1; Protein NH2-terminal glutamine deamidase; N-terminal Gln amidase; Nt(Q)-amidase; WDYHV motif-containing protein 1; anti-WDYHV1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G Purified
• Sequence Length: 145

• Applicable Applications for anti-WDYHV1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Recommended dilution: WB:1:500-2000
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Protein N-terminal glutamine amidohydrolase protein
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: WDYHV1 antibody at 6ug/mlLane 1: Mouse heart tissueLane 2: MCF7 whole cell lysateLane 3: U251 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 24,17 kDaObserved band size: 24 kDa)

Related Product Information for anti-WDYHV1 antibody

Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 545687946
• NCBI GeneID: 55093
• NCBI Accession #: NP_001269953.1
• NCBI GenBank Nucleotide #: NM_001283024.1
• UniProt Accession #: Q96HA8; Q9NW95; B4DE68
• Molecular Weight: 16,736 Da
• NCBI Official Full Name: protein N-terminal glutamine amidohydrolase isoform 2
• NCBI Official Synonym Full Names: WDYHV motif containing 1
• NCBI Official Symbol: WDYHV1
• NCBI Official Synonym Symbols: C8orf32
• NCBI Protein Information: protein N-terminal glutamine amidohydrolase
• UniProt Protein Name: Protein N-terminal glutamine amidohydrolase
• UniProt Gene Name: WDYHV1
• UniProt Synonym Gene Names: C8orf32; NTAQ1; N-terminal Gln amidase; Nt(Q)-amidase
• UniProt Entry Name: NTAQ1_HUMAN

Uniprot Description

WDYHV1: Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N- terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C- terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine. Belongs to the NTAQ1 family.

Protein type: EC 3.5.1.-

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: cytosol; nucleus

Molecular Function: protein binding

Biological Process: protein modification process