Western Blot (WB)
(Western blot analysis of WDR82 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). WDR82 (arrow) was detected using the purified Pab.)
Rabbit WDR82 Polyclonal Antibody | anti-WDR82 antibody
WDR82 Antibody (N-term)
Western Blot (WB)
(Western blot analysis of WDR82 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). WDR82 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of WDR82 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). WDR82 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(WDR82 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR82 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(WDR82 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR82 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(WDR82 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(WDR82 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Lee, J.H., et al. Mol. Cell. Biol. 28(2):609-618(2008)
Higa, L.A., et al. Nat. Cell Biol. 8(11):1277-1283(2006)
NCBI and Uniprot Product Information
NCBI Description
TMEM113 (WDR82) is a component of the mammalian SET1A (MIM 611052)/SET1B (MIM 611055) histone H3-Lys4 methyltransferase complexes (Lee and Skalnik, 2005 [PubMed 16253997]; Lee et al., 2007 [PubMed 17355966]).[supplied by OMIM, Jul 2010]
Uniprot Description
WDR82: a WD repeat-containing protein. A regulatory component of the histone H3K4 methylatransferase SET1 complex implicated in tethering this complex to transcriptional start sites of active genes. Facilitates histone H3K4 methylation via recruitment of the SETD1A or SETD1B to phospho-S5 in the C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Component of the SET1 complex, composed of at least the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA, ¿B, or -C. Associated with multiple protein complexes including an RNA polymerase II complex, MLL3/MLL4 complex and a chaperonin-containing TCP1 complex. Interacts with CUL4B. Interacts with RBBP5 and SETD1B. Interacts with SETD1A (via RRM domain). Interacts with POLR2B. Interacts with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). SETD1A enhances its interaction with POLR2A. Interacts with PPP1R10/PNUTS. Interacts with PPP1CA in the presence of PPP1R10/PNUTS.
Protein type: Methyltransferase
Chromosomal Location of Human Ortholog: 3p21.2
Cellular Component: histone methyltransferase complex; chromatin
Molecular Function: protein binding; histone lysine N-methyltransferase activity (H3-K4 specific); chromatin binding
Biological Process: histone H3-K4 methylation
Research Articles on WDR82
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Product Notes
The WDR82 wdr82 (Catalog #AAA9201610) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The WDR82 Antibody (N-term) reacts with Human (Predicted Reactivity: Xenopus, Chicken, Zebrafish, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's WDR82 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the WDR82 wdr82 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WDR82, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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