Rabbit anti-Human WDR82 Polyclonal Antibody | anti-WDR82 antibody

WDR82, NT (WDR82, WDR82A, WD repeat-containing protein 82, Swd2) (APC)

Cat. Num. AAA6363424

• Gene Names: WDR82; SWD2; MST107; WDR82A; MSTP107; PRO2730; TMEM113; PRO34047
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: WDR82; Polyclonal Antibody; NT (WDR82; WDR82A; WD repeat-containing protein 82; Swd2) (APC); Swd2; anti-WDR82 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-WDR82 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: WDR82 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~31 amino acids from the N-terminal region of human WDR82.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of WDR82 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). WDR82 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(WDR82 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR82 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(WDR82 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-WDR82 antibody

WDR82 is a component of the mammalian SET1A (MIM 611052)/SET1B (MIM 611055) histone H3-Lys4 methyltransferase complexes.

Product Categories/Family for anti-WDR82 antibody

Antibodies; WD Repeat Proteins

References

Wu, M., et al. Mol. Cell. Biol. 28(24):7337-7344(2008). Lee, J.H., et al. Mol. Cell. Biol. 28(2):609-618(2008). Higa, L.A., et al. Nat. Cell Biol. 8(11):1277-1283(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 147904340
• NCBI GeneID: 80335
• NCBI Accession #: NP_079498.2
• NCBI GenBank Nucleotide #: NM_025222.3
• UniProt Accession #: Q6UXN9; Q8TEB2; A8K5R5
• Molecular Weight: 35,079 Da
• NCBI Official Full Name: WD repeat-containing protein 82
• NCBI Official Synonym Full Names: WD repeat domain 82
• NCBI Official Symbol: WDR82
• NCBI Official Synonym Symbols: SWD2; MST107; WDR82A; MSTP107; PRO2730; TMEM113; PRO34047
• NCBI Protein Information: WD repeat-containing protein 82; transmembrane protein 113
• UniProt Protein Name: WD repeat-containing protein 82
• Protein Family: WD repeat-containing protein
• UniProt Gene Name: WDR82
• UniProt Synonym Gene Names: TMEM113; WDR82A
• UniProt Entry Name: WDR82_HUMAN

NCBI Description

TMEM113 (WDR82) is a component of the mammalian SET1A (MIM 611052)/SET1B (MIM 611055) histone H3-Lys4 methyltransferase complexes (Lee and Skalnik, 2005 [PubMed 16253997]; Lee et al., 2007 [PubMed 17355966]).[supplied by OMIM, Jul 2010]

Uniprot Description

WDR82: a WD repeat-containing protein. A regulatory component of the histone H3K4 methylatransferase SET1 complex implicated in tethering this complex to transcriptional start sites of active genes. Facilitates histone H3K4 methylation via recruitment of the SETD1A or SETD1B to phospho-S5 in the C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Component of the SET1 complex, composed of at least the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA, ¿B, or -C. Associated with multiple protein complexes including an RNA polymerase II complex, MLL3/MLL4 complex and a chaperonin-containing TCP1 complex. Interacts with CUL4B. Interacts with RBBP5 and SETD1B. Interacts with SETD1A (via RRM domain). Interacts with POLR2B. Interacts with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). SETD1A enhances its interaction with POLR2A. Interacts with PPP1R10/PNUTS. Interacts with PPP1CA in the presence of PPP1R10/PNUTS.

Protein type: Methyltransferase

Chromosomal Location of Human Ortholog: 3p21.2

Cellular Component: histone methyltransferase complex; chromatin

Molecular Function: protein binding; histone lysine N-methyltransferase activity (H3-K4 specific); chromatin binding

Biological Process: histone H3-K4 methylation