Typical Testing Data/Standard Curve (for reference only)
Human Proto-oncogene Wnt-1, WNT1 ELISA Kit | WNT1 elisa kit
Human Proto-oncogene Wnt-1, WNT1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human WNT1 antibody. WNT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human WNT1 Antibody is added and binds to WNT1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated WNT1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human WNT1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI Description
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]
Uniprot Description
WNT1: Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Belongs to the Wnt family.
Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Oncoprotein; Cell development/differentiation
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; Golgi lumen; endoplasmic reticulum lumen; cytoplasm; extracellular region; plasma membrane
Molecular Function: protein domain specific binding; frizzled binding; cytokine activity; receptor agonist activity
Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; ubiquitin-dependent SMAD protein catabolic process; diencephalon development; negative regulation of BMP signaling pathway; neuron differentiation; cerebellum formation; positive regulation of fibroblast proliferation; cell-cell signaling; midbrain development; positive regulation of cell proliferation; midbrain-hindbrain boundary maturation during brain development; response to wounding; positive regulation of Notch signaling pathway; myoblast fusion; neuron fate determination; inner ear morphogenesis; organ regeneration; cell fate commitment; Wnt receptor signaling pathway; embryonic axis specification; DNA damage response, signal transduction; negative regulation of fat cell differentiation; central nervous system morphogenesis; ureteric bud branching; forebrain anterior/posterior pattern formation; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell-cell adhesion; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; Spemann organizer formation
Disease: Bone Mineral Density Quantitative Trait Locus 16; Osteogenesis Imperfecta, Type Xv
Research Articles on WNT1
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Product Notes
The Human WNT1 wnt1 (Catalog #AAA1606890) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1606890 ELISA Kit recognizes Human WNT1. It is sometimes possible for the material contained within the vial of "Proto-oncogene Wnt-1, WNT1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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