Western Blot (WB)
(WDR32 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the WDR32 antibody detected the WDR32 protein (arrow).)
Rabbit anti-Human WDR32 Polyclonal Antibody | anti-DCAF10 antibody
WDR32, NT (DCAF10, WDR32, DDB1- and CUL4-associated factor 10, WD repeat-containing protein 32)
Gene Names
DCAF10; WDR32
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
WDR32; Polyclonal Antibody; NT (DCAF10; DDB1- and CUL4-associated factor 10; WD repeat-containing protein 32); Anti -WDR32; anti-DCAF10 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-DCAF10 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
WDR32 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-148 amino acids from the N-terminal region of human WDR32.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(WDR32 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the WDR32 antibody detected the WDR32 protein (arrow).)
Western Blot (WB)
(WDR32 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the WDR32 antibody detected the WDR32 protein (arrow).)
Immunohistochemistry (IHC)
(WDR32 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR32 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(WDR32 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR32 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(WDR32 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(WDR32 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-DCAF10 antibody
DCAF10 may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
Product Categories/Family for anti-DCAF10 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
60,582 Da
NCBI Official Full Name
WDR32 protein, partial
NCBI Official Synonym Full Names
DDB1 and CUL4 associated factor 10
NCBI Official Symbol
DCAF10
NCBI Official Synonym Symbols
WDR32
NCBI Protein Information
DDB1- and CUL4-associated factor 10; WD repeat domain 32; WD repeat-containing protein 32
UniProt Protein Name
DDB1- and CUL4-associated factor 10
Protein Family
UniProt Gene Name
DCAF10
UniProt Synonym Gene Names
WDR32
UniProt Entry Name
DCA10_HUMAN
Uniprot Description
WDR32: May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. Belongs to the WD repeat DCAF10 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 9p13.2
Biological Process: protein ubiquitination
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Product Notes
The DCAF10 dcaf10 (Catalog #AAA6004074) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WDR32, NT (DCAF10, WDR32, DDB1- and CUL4-associated factor 10, WD repeat-containing protein 32) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WDR32 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the DCAF10 dcaf10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WDR32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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