Rabbit MEIS2 Polyclonal Antibody | anti-MEIS2 antibody

MEIS2 Antibody

Cat. Num. AAA9611892

• Gene Names: MEIS2; MRG1; CPCMR; HsT18361
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MEIS2; Polyclonal Antibody; MEIS2 Antibody; Homeobox protein Meis2; HsT18361; Meis (mouse) homolog 2; Meis homeobox 2; Meis homolog 2; Meis1 myeloid ecotropic viral integration site 1 homolog 2 (mouse); Meis1 myeloid ecotropic viral integration site 1 homolog 2; Meis1 related gene 1; Meis1 related protein 1; Meis1-related protein 1; MEIS2_HUMAN; MGC2820; MRG1; TALE homeobox protein Meis2; anti-MEIS2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MEIS2 Antibody detects endogenous levels of MEIS2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 381

• Applicable Applications for anti-MEIS2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human MEIS2.
• Tissue Specificity: Expressed in various tissues. Expressed at high level in the lymphoid organs of hematopoietic tissues. Also expressed in some regions of the brain, such as the putamen.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from ?Jurkat cells, using MEIS2 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-MEIS2 antibody

Description: Involved in transcriptional regulation. Binds to HOX or PBX proteins to form dimers, or to a DNA-bound dimer of PBX and HOX proteins and thought to have a role in stabilization of the homeoprotein-DNA complex. Isoform 3 is required for the activity of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element; MEIS2 is not involved in complex DNA-binding. Probably in complex with PBX1, is involved in transcriptional regulation by KLF4. Isoform 3 and isoform 4 can bind to a EPHA8 promoter sequence containing the DNA motif 5'-CGGTCA-3'; in cooperation with a PBX protein (such as PBX2) is proposed to be involved in the transcriptional activation of EPHA8 in the developing midbrain. May be involved in regulation of myeloid differentiation. Can bind to the DNA sequence 5'-TGACAG-3'in the activator ACT sequence of the D(1A) dopamine receptor (DRD1) promoter and activate DRD1 transcription; isoform 5 cannot activate DRD1 transcription.

Subunit Structure: Monomer and homodimer (PubMed:26550823). Heterodimer with HOXB13 (Ref. 16). Isoform 2 interacts with TLX1. Isoform 3 interacts with HOXA13 and PBX1 isoform PBX1b. Isoform 4 interacts with SP1, SP3 and KLF4. Isoform 4 and isoform 5 interact with PBX1 isoform PBX1a; the interaction partially relieves MEIS2 autoinhibition. Isoform 3 also known as MEIS2b is part of a PDX1:PBX1b:Meis2B complex; Meis2B is recruited by PBX1b and can be replaced by isoform 4 in a small fraction of complexes. Can form trimeric complexes including HOXB8 and PBX2 or PBX3.

Similarity: Belongs to the TALE/MEIS homeobox family.

NCBI and Uniprot Product Information

• NCBI GI #: 112180329
• NCBI GeneID: 4212
• UniProt Accession #: O14770
• Molecular Weight: Observed: 56 kDa; Predicted: 52 kDa
• NCBI Official Full Name: Meis homeobox 2
• NCBI Official Synonym Full Names: Meis homeobox 2
• NCBI Official Symbol: MEIS2
• NCBI Official Synonym Symbols: MRG1; CPCMR; HsT18361
• NCBI Protein Information: homeobox protein Meis2
• UniProt Protein Name: Homeobox protein Meis2
• Protein Family: Homeobox protein
• UniProt Gene Name: MEIS2
• UniProt Synonym Gene Names: MRG1
• UniProt Entry Name: MEIS2_HUMAN

NCBI Description

This gene encodes a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. TALE homeobox proteins are highly conserved transcription regulators, and several members have been shown to be essential contributors to developmental programs. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MEIS2: a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. TALE homeobox proteins are highly conserved transcription regulators, and several members have been shown to be essential contributors to developmental programs. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription cofactor activity; transcription factor activity; transcription factor binding; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of myeloid cell differentiation; eye development; response to mechanical stimulus; pancreas development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter