Rabbit anti-Human, Mouse WDFY2 Polyclonal Antibody | anti-WDFY2 antibody

WDFY2 Antibody

Cat. Num. AAA9417315

• Gene Names: WDFY2; PROF; WDF2; ZFYVE22
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Antigen affinity purification
• Synonyms: WDFY2; Polyclonal Antibody; WDFY2 Antibody; PROF; WDF2; ZFYVE22; anti-WDFY2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total WDFY2 protein.
• Purity/Purification: Antigen affinity purification
• Form/Format: Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Concentration: 0.9mg/ml (varies by lot)
• Sequence Length: 400

• Applicable Applications for anti-WDFY2 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Western Blot: 1:200-1000; Immunohistochemistry: 1: 30-150
• Immunogen: Fusion protein of human WDFY2
• Immunogen Type: Protein
• Preparation and Storage: Store at -20 degree C

SDS-Page

(Gel: 8%SDS-PAGE Lysate: 40 ug, Lane: Mouse kidney tissue lysate, Primary antibody:WDFY2 antibody at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15 seconds)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using WDFY2 Antibody at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200))

Related Product Information for anti-WDFY2 antibody

WD repeat and FYVE domain-containing protein 2 (WDFY2), also known as WDF2 and ZFYVE22, is a 400 amino acid protein that localizes to a set of small endosomes that are found within 100 nm from the plasma membrane. Highly conserved between species, WDFY2 consists of one FYVE-type zinc finger and seven WD repeats. The FYVE domain is a cysteine-rich domain of about 70 amino acids. Its primary role is to target signal-transducing proteins to cell membranes through binding to the membrane lipid phosphatidylinositol-3-phosphate with high specificity. WD-repeats are generally found in clusters of seven. They have no intrinsic catalytic activity, but they serve as a platform for protein-protein interactions. WDFY2 is suspected to play a critical role in the endocytic pathway.

Product Categories/Family for anti-WDFY2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 16418439
• NCBI GeneID: 115825
• NCBI Accession #: NP_443182.1
• NCBI GenBank Nucleotide #: NM_052950.3
• UniProt Accession #: Q96P53; Q96CS1; B1AL86
• Molecular Weight: 45,154 Da
• NCBI Official Full Name: WD repeat and FYVE domain-containing protein 2
• NCBI Official Synonym Full Names: WD repeat and FYVE domain containing 2
• NCBI Official Symbol: WDFY2
• NCBI Official Synonym Symbols: PROF; WDF2; ZFYVE22
• NCBI Protein Information: WD repeat and FYVE domain-containing protein 2
• UniProt Protein Name: WD repeat and FYVE domain-containing protein 2
• UniProt Gene Name: WDFY2
• UniProt Synonym Gene Names: WDF2; ZFYVE22; Prof
• UniProt Entry Name: WDFY2_HUMAN

NCBI Description

This gene encodes a protein that contains two WD domains and an FYVE zinc finger region. The function of this gene is unknown. An alternatively spliced transcript variant of this gene may exist. [provided by RefSeq, Jul 2008]

Uniprot Description

WDFY2: Acts in an adapter protein-like fashion to mediate the interaction between the kinase PRKCZ and its substrate VAMP2 and increases the PRKCZ-dependent phosphorylation of VAMP2 (PubMed:17313651). Positively regulates adipocyte differentiation, by facilitating the phosphorylation and thus inactivation of the anti-adipogenetic transcription factor FOXO1 by the kinase AKT1 (PubMed:18388859). Plays a role in endosomal control of AKT2 signaling; required for insulin-stimulated AKT2 phosphorylation and glucose uptake and insulin-stimulated phosphorylation of AKT2 substrates. Participates in transferrin receptor endocytosis (PubMed:16873553). {ECO:0000250|UniProtKB:Q8BUB4, ECO:0000269|PubMed:16873553, ECO:0000269|PubMed:17313651, ECO:0000269|PubMed:18388859}. Homodimer (PubMed:16792529). Interacts (via WD repeats 1- 3) with AKT1, AKT2, PRKCZ and PRKCI (PubMed:16792529). Interacts with VAMP2 (PubMed:17313651). Forms a complex with VAMP2 and PRKCZ (PubMed:17313651). Interacts with FOXO1. Forms a complex with AKT1 and FOXO1. {ECO:0000250|UniProtKB:Q8BUB4, ECO:0000269|PubMed:16792529, ECO:0000269|PubMed:17313651}

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: early endosome; vesicle

Molecular Function: protein binding

Biological Process: positive regulation of fat cell differentiation; positive regulation of protein amino acid phosphorylation