Typical Testing Data/Standard Curve (for reference only)
Human Prolyl endopeptidase (PREP) ELISA Kit | PREP elisa kit
Human Prolyl endopeptidase (PREP) ELISA Kit
Gene Names
PREP; PE; PEP
Reactivity
Human
Synonyms
Prolyl endopeptidase (PREP); Human Prolyl endopeptidase (PREP) ELISA Kit; PREP elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of PREP. No significant cross-reactivity or interference between PREP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PREP and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PREP elisa kit
Intended Uses: This PREP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PREP. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: PREP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PREP antibody and an PREP-HRP conjugate. The assay sample and buffer are incubated together with PREP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PREP concentration since PREP from samples and PREP-HRP conjugate compete for the anti-PREP antibody binding site. Since the number of sites is limited, as more sites are occupied by PREP from the sample, fewer sites are left to bind PREP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PREP concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PREP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PREP antibody and an PREP-HRP conjugate. The assay sample and buffer are incubated together with PREP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PREP concentration since PREP from samples and PREP-HRP conjugate compete for the anti-PREP antibody binding site. Since the number of sites is limited, as more sites are occupied by PREP from the sample, fewer sites are left to bind PREP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PREP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80,700 Da
NCBI Official Full Name
prolyl endopeptidase
NCBI Official Synonym Full Names
prolyl endopeptidase
NCBI Official Symbol
PREP
NCBI Official Synonym Symbols
PE; PEP
NCBI Protein Information
prolyl endopeptidase; prolyl oligopeptidase; post-proline cleaving enzyme; dJ355L5.1 (prolyl endopeptidase)
UniProt Protein Name
Prolyl endopeptidase
Protein Family
UniProt Gene Name
PREP
UniProt Synonym Gene Names
PEP; PE
UniProt Entry Name
PPCE_HUMAN
NCBI Description
The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides. [provided by RefSeq, Jul 2008]
Uniprot Description
PREP: Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Belongs to the peptidase S9A family.
Protein type: EC 3.4.21.26; Protease
Chromosomal Location of Human Ortholog: 6q22
Cellular Component: membrane; cytoplasm; nucleus
Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: proteolysis
Research Articles on PREP
Similar Products
Product Notes
The Human PREP prep (Catalog #AAA7250306) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7250306 ELISA Kit recognizes Human PREP. It is sometimes possible for the material contained within the vial of "Prolyl endopeptidase (PREP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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