Rabbit WAVE1 Polyclonal Antibody | anti-WAVE1 antibody
WAVE1 Antibody
Gene Names
WASF1; WAVE; SCAR1; WAVE1
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
WAVE1; Polyclonal Antibody; WAVE1 Antibody; ;WASP family; verprolin homology domain-containing protein 1; WASP family member 1; FLJ31482; homology of dictyostelium scar 1; KIAA0269; Protein WAVE-1; Protein WAVE1; scar; Dictyostelium; homology of; 1; SCAR1; Similar to a plant extensin like protein; Verprolin homology domain containing protein 1; Verprolin homology domain-containing protein 1; WAS protein family; member 1; Wasf1; WASF1_HUMAN; WASL; WASP family 1; WASP family protein member 1; WASP family; verprolin homology domain-containing protein; WAVE; Wiskott Aldrich syndrome protein family member 1; Wiskott-Aldrich syndrome protein family member 1; anti-WAVE1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
WAVE1 Antibody detects endogenous levels of total WAVE1.
Tissue Specificity: Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.
Tissue Specificity: Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-WAVE1 antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human WAVE1.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated on tyrosine residues by ABL1 and dephosphorylated by PTPN12.
Subunit Structure
Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Interacts with SHANK3; the interaction mediates the association of SHANK3 with the WAVE1 complex. Interacts with ABI1 (via N-terminus). Interacts with SORBS2; this interaction greatly enhances phosphorylation by ABL1 and dephosphorylation by PTPN12 and might mediate partial to focal adhesion sites.
Similarity
Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.Belongs to the SCAR/WAVE family.
Subcellular Location
Cytoplasm>Cytoskeleton. Cell junction>Synapse. Cell junction>Focal adhesion.
Note: Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas (PubMed:9889097). Partial translocation to focal adhesion sites might be mediated by interaction with SORBS2 (PubMed:18559503). In neurons, colocalizes with activated NTRK2 after BDNF addition in endocytic sites through the association with TMEM108 (By similarity).
Note: Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas (PubMed:9889097). Partial translocation to focal adhesion sites might be mediated by interaction with SORBS2 (PubMed:18559503). In neurons, colocalizes with activated NTRK2 after BDNF addition in endocytic sites through the association with TMEM108 (By similarity).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Related Product Information for anti-WAVE1 antibody
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,652 Da
NCBI Official Full Name
wiskott-Aldrich syndrome protein family member 1
NCBI Official Synonym Full Names
WAS protein family, member 1
NCBI Official Symbol
WASF1
NCBI Official Synonym Symbols
WAVE; SCAR1; WAVE1
NCBI Protein Information
wiskott-Aldrich syndrome protein family member 1; protein WAVE-1; WASP family protein member 1; homology of dictyostelium scar 1; verprolin homology domain-containing protein 1
UniProt Protein Name
Wiskott-Aldrich syndrome protein family member 1
UniProt Gene Name
WASF1
UniProt Synonym Gene Names
KIAA0269; SCAR1; WAVE1; WASP family protein member 1
UniProt Entry Name
WASF1_HUMAN
NCBI Description
The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
WAVE1: Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Belongs to the SCAR/WAVE family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 6q21
Cellular Component: cytoskeleton; synapse; cell junction; actin cytoskeleton
Molecular Function: protein binding; Rac GTPase binding; protein complex binding; actin binding
Biological Process: actin filament polymerization; Rac protein signal transduction; protein complex assembly; cell motility
Research Articles on WAVE1
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Product Notes
The WAVE1 wasf1 (Catalog #AAA9613585) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WAVE1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's WAVE1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the WAVE1 wasf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WAVE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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