Rabbit anti-Human WASP, N- Polyclonal Antibody | anti-WAS antibody

WASP, N-, phosphorylated (Tyr-256) (WAS, THC, IMD2, WASP, Eczema-thrombocytopenia)

Cat. Num. AAA621546

• Gene Names: WAS; THC; IMD2; SCNX; THC1; WASP
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: WASP; N-; Polyclonal Antibody; phosphorylated (Tyr-256) (WAS; THC; IMD2; Eczema-thrombocytopenia); Anti -WASP; anti-WAS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes a 65kD protein corresponding to the molecular mass of human phosphorylated N-WASP. A similar band is observed in pervanadate treated HeLa and endothelial cells. Weak bands are also observed at a higher MW after pervanadate treatment, may be due to weak cross reactivity with phosphotyrosine.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-WAS antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunocytochemistry.; Dilution: Western Blot: 1:10,000; ELISA: 1:20,000; Immunocytochemistry: 1:100
• Immunogen: Synthetic peptide corresponding to amino acid residues around phosphorylated tyrosine 256 of human N-WASP, coupled to BSA. Sequence homology: Two amino acid sequence difference in the region surrounding WASP tyrosine 291.
• Positive Control: Pervanadate treated A431, HeLa and endothelial cells.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of control and pervanadate-treated A431 cells (20 µg/lane). Blots were probed with MBS621546.)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of N-WASP in control and pervanadate-treated A431 cells. The cells were labeled with MBS621546 or rabbit polyclonal N-WASP (Tyr-256) antibodies, then the antibodies were detected using appropriate secondary antibody.)

Related Product Information for anti-WAS antibody

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with higher homology in the functional regions of these proteins. Phosphorylation at serine and tyrosine residues regulates the activity of both proteins. WASP is tyrosine phosphorylated at tyrosine 291 after antigen receptor activation in B-cells and collagen stimulation of platelets. Phosphorylation of the analogous site in N-WASP (Tyr-256) stimulates its activity, reduces nuclear N-WASP, and is required for neurite extension.

Product Categories/Family for anti-WAS antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 4507909
• NCBI GeneID: 7454
• NCBI Accession #: NP_000368.1
• NCBI GenBank Nucleotide #: NM_000377.2
• UniProt Accession #: P42768; Q9BU11; Q9UNJ9
• Molecular Weight: 52,913 Da
• NCBI Official Full Name: wiskott-Aldrich syndrome protein
• NCBI Official Synonym Full Names: Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
• NCBI Official Symbol: WAS
• NCBI Official Synonym Symbols: THC; IMD2; SCNX; THC1; WASP
• NCBI Protein Information: wiskott-Aldrich syndrome protein; OTTHUMP00000032395; thrombocytopenia 1 (X-linked)
• UniProt Protein Name: Wiskott-Aldrich syndrome protein
• Protein Family: Wiskott-Aldrich syndrome protein
• UniProt Gene Name: WAS
• UniProt Synonym Gene Names: IMD2
• UniProt Entry Name: WASP_HUMAN

NCBI Description

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq]

Uniprot Description

WASP: a member of the Wiskott-Aldrich syndrome (WAS) family of proteins. A cytoplasmic protein expressed exclusively in hematopoietic cells. Transduces signals from surface receptors to the actin cytoskeleton. Associates with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Mutated in Wiskott-Aldrich syndrome, a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xp11.4-p11.21

Cellular Component: vesicle membrane; intercellular junction; cytosol; actin cytoskeleton

Molecular Function: identical protein binding; protein binding; phospholipase binding; actin binding; protein kinase binding; SH3 domain binding

Biological Process: epidermis development; T cell activation; actin filament-based movement; defense response; endosome transport; T cell receptor signaling pathway; actin filament polymerization; regulation of catalytic activity; actin polymerization and/or depolymerization; innate immune response; immune response; protein complex assembly; blood coagulation

Disease: Thrombocytopenia 1; Neutropenia, Severe Congenital, X-linked