Rabbit anti-Human WASF2 Polyclonal Antibody | anti-WASF2 antibody

WASF2, ID (WASF2, WAVE2, Wiskott-Aldrich syndrome protein family member 2, Protein WAVE-2, Verprolin homology domain-containing protein 2)

Cat. Num. AAA646218

• Gene Names: WASF2; IMD2; SCAR2; WASF4; WAVE2; dJ393P12.2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: WASF2; Polyclonal Antibody; ID (WASF2; WAVE2; Wiskott-Aldrich syndrome protein family member 2; Protein WAVE-2; Verprolin homology domain-containing protein 2); Anti -WASF2; anti-WASF2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-WASF2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: WASF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from the Central region of human WASF2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WASF2 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the WASF2 antibody detected the WASF2 protein (arrow).)

Related Product Information for anti-WASF2 antibody

This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location (PMID:10381382) has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X.

Product Categories/Family for anti-WASF2 antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 319655728
• NCBI GeneID: 10163
• NCBI Accession #: NP_001188333.1
• NCBI GenBank Nucleotide #: NM_001201404.1
• UniProt Accession #: Q9Y6W5; O60794; Q9UDY7; B4DZN0
• Molecular Weight: 54,284 Da
• NCBI Official Full Name: wiskott-Aldrich syndrome protein family member 2 isoform 2
• NCBI Official Synonym Full Names: WAS protein family, member 2
• NCBI Official Symbol: WASF2
• NCBI Official Synonym Symbols: IMD2; SCAR2; WASF4; WAVE2; dJ393P12.2
• NCBI Protein Information: wiskott-Aldrich syndrome protein family member 2; protein WAVE-2; WASP family protein member 2; WASP family protein member 4; WASP family Verprolin-homologous protein 2; verprolin homology domain-containing protein 2; suppressor of cyclic-AMP receptor (WASP-family); putative Wiskott-Aldrich syndrome protein family member 4
• UniProt Protein Name: Wiskott-Aldrich syndrome protein family member 2
• Protein Family: Wiskott-Aldrich syndrome protein family
• UniProt Gene Name: WASF2
• UniProt Synonym Gene Names: WAVE2; WASP family protein member 2
• UniProt Entry Name: WASF2_HUMAN

NCBI Description

This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location (PMID:10381382) has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Function: Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Ref.1 Ref.9

Subunit structure: Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. Directly interacts with BRK1. Ref.1 Ref.5 Ref.6 Ref.7 Ref.9

Subcellular location: Cytoplasm › cytoskeleton

By similarity. Cell projection › lamellipodium

By similarity. Note: At the interface between the lamellipodial actin meshwork and the membrane

By similarity.

Tissue specificity: Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle. Ref.1

Domain: Binds and activates the Arp2/3 complex via the C-terminal domain. Interacts with actin via the WH2 domain. Ref.1 Ref.9

Sequence similarities: Belongs to the SCAR/WAVE family.Contains 1 WH2 domain.