Rabbit anti-Human P2RY6 Polyclonal Antibody | anti-P2RY6 antibody

Anti-P2RY6 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103679

• Gene Names: P2RY6; P2Y6
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: P2RY6; Polyclonal Antibody; Anti-P2RY6 Antibody; Rabbit Polyclonal; P2RY6 Antibody; Rabbit PAb; Antigen Affinity Purified; pyrimidinergic receptor P2Y; G-protein coupled; 6; Anti-P2Y6 Antibody; Anti-PP2891 Antibody; anti-P2RY6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human P2RY6
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-P2RY6 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:500-1:2000; IP: 5-10uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the Human P2RY6
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human P2RY6, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-P2RY6 rabbit polyclonal antibody at 1:500 dilution Lane A: A549 Whole Cell Lysate Lane B: 293 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:36 kDa Observed band size:36 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human P2RY6 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human P2RY6 in human ovarian cancer with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(P2RY6 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 uL anti-P2RY6 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-P2RY6 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa Observed band size :35 kDa)

Related Product Information for anti-P2RY6 antibody

P2RY6 (Pyrimidinergic Receptor P2Y6) is a Protein Coding gene. The P2RY6 gene, located on 11q13.4, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, and zebrafish. The product of this gene belongs to the family of P2 receptors, which are activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, which is a G-protein coupled receptor, is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. It is proposed that this receptor mediates inflammatory responses. Among its related pathways are RET signaling and Signaling by GPCR.

References

Obba S, et al. (2015) The prkaa1/ampkalpha1 pathway triggers autophagy during csf1-induced human monocyte differentiation and is a potential target in cmml. Autophagy 11 (7): 1114-1129.Nakano M, et al. (2017) Udp/p2y6 receptor signaling regulates ige-dependent degranulation in human basophils. Allergol Int 66 (4): 574-580.Hao Y, et al. (2014) P2y6 receptor-mediated proinflammatory signaling in human bronchial epithelia. PLoS One 9 (9): e106235.

NCBI and Uniprot Product Information

• NCBI GI #: 2495018
• NCBI GeneID: 5031
• UniProt Accession #: Q15077; Q15754
• Molecular Weight: 328
• NCBI Official Full Name: P2Y purinoceptor 6
• NCBI Official Synonym Full Names: pyrimidinergic receptor P2Y, G-protein coupled, 6
• NCBI Official Symbol: P2RY6
• NCBI Official Synonym Symbols: P2Y6
• NCBI Protein Information: P2Y purinoceptor 6; P2Y6 receptor; P2 purinoceptor; G-coupled nucleotide receptor
• UniProt Protein Name: P2Y purinoceptor 6
• Protein Family: P2Y purinoceptor
• UniProt Gene Name: P2RY6
• UniProt Synonym Gene Names: P2Y6
• UniProt Entry Name: P2RY6_HUMAN

NCBI Description

The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, which is a G-protein coupled receptor, is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. It is proposed that this receptor mediates inflammatory responses. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Mar 2013]

Uniprot Description

P2Y6: Receptor for extracellular UDP > UTP > ATP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: integral to plasma membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; UDP-activated nucleotide receptor activity; purinergic nucleotide receptor activity, G-protein coupled

Biological Process: transepithelial chloride transport; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of smooth muscle cell migration