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Western Blot (WB) (Western BlotPositive WB detected in: Jurkat whole cell lysate,Hela whole cell lysateAll lanes: WAPL antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 133,138,143 KDaObserved band size: 133KDa)

Rabbit anti-Human WAPL Polyclonal Antibody | anti-WAPL antibody

WAPL Antibody

Gene Names
WAPL; FOE; WAPAL; KIAA0261
Reactivity
Human
Applications
ELISA
Purity
>95%, Protein G purified
Synonyms
WAPL; Polyclonal Antibody; WAPL Antibody; Wings apart-like protein homolog; Friend of EBNA2 protein; WAPL cohesin release factor; anti-WAPL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
1184
Applicable Applications for anti-WAPL antibody
ELISA (EIA)
Immunogen
Recombinant Human Wings apart-like protein homolog protein (210-402AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-82118 / sc-82120 / sc-82121
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Jurkat whole cell lysate,Hela whole cell lysateAll lanes: WAPL antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 133,138,143 KDaObserved band size: 133KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Jurkat whole cell lysate,Hela whole cell lysateAll lanes: WAPL antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 133,138,143 KDaObserved band size: 133KDa)

Western Blot (WB)

(Western BlotPositive WB detected in: MCF-7 whole cell lysate,Jurkat whole cell lysate,Hela whole cell lysate,K562 whole cell lysateAll lanes: WAPL antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 133,138,143 KDaObserved band size: 133 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: MCF-7 whole cell lysate,Jurkat whole cell lysate,Hela whole cell lysate,K562 whole cell lysateAll lanes: WAPL antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 133,138,143 KDaObserved band size: 133 KDa)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of Hela cells using MBS7050611 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunohistochemistry (IHC) (Immunofluorescent analysis of Hela cells using MBS7050611 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-WAPL antibody
Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
142,525 Da
NCBI Official Full Name
wings apart-like protein homolog isoform 2
NCBI Official Synonym Full Names
WAPL cohesin release factor
NCBI Official Symbol
WAPL
NCBI Official Synonym Symbols
FOE; WAPAL; KIAA0261
NCBI Protein Information
wings apart-like protein homolog
UniProt Protein Name
Wings apart-like protein homolog
Protein Family
UniProt Gene Name
WAPL
UniProt Entry Name
WAPL_HUMAN

Uniprot Description

WAPL: a chromatin-associated protein partner of Epstein-Barr nuclear protein 2. Associated with cervical carcinogenesis and tumor progression.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 10q23.2

Cellular Component: chromatin; chromosome; chromosome, pericentric region; cohesin complex; cytoplasm; cytosol; intracellular membrane-bound organelle; microtubule cytoskeleton; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: meiotic chromosome segregation; mitotic sister chromatid cohesion; negative regulation of DNA replication; negative regulation of sister chromatid cohesion; sister chromatid cohesion

Research Articles on WAPL

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Product Notes

The WAPL wapl (Catalog #AAA7050611) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WAPL Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WAPL can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the WAPL wapl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WAPL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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