Rabbit anti-Human VSIG4 Polyclonal Antibody | anti-VSIG4 antibody

Anti-VSIG4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106231

• Gene Names: VSIG4; CRIg; Z39IG
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: VSIG4; Polyclonal Antibody; Anti-VSIG4 Antibody; Rabbit Polyclonal; VSIG4 Antibody; Rabbit PAb; Antigen Affinity Purified; V-set and immunoglobulin domain containing 4; Anti-CRIg Antibody; Anti-Z39IG Antibody; anti-VSIG4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human VSIG4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-VSIG4 antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000
• Immunogen: Recombinant Human VSIG4 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human VSIG4 (rh VSIG4). VSIG4 specific IgG was purified by Human VSIG4 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human VSIG4 in human stomach with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human VSIG4 in human placenta with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-VSIG4 antibody

VSIG4 (V-set and immunoglobulin domain containing 4), also known as complement receptor of the immunoglobulin superfamily (CRIg) and Z39Ig, is a type I transmembrane glycoprotein. It is a B7 family-related protein and an Ig superfamily member. In contrast to the B7 family members which contain two IgG domains, VSIG4 contains one complete V-type I g domain and a truncated C-type I g domain. VSIG4 is exclusively expressed on tissue resident macrophages and binds to multimers of C3b and iC3b that are covalently attached to particle surfaces. No VSIG4 expression appears to be present in T and B cells. VSIG4 functions as a negative regulator of T cell activation, and may be involved in the maintenance of peripheral T cell tolerance, and is also identified as a potent suppressor of established inflammation. Mouse VSIG4 is synthesized as a 28 amino acid precursor that contains a signal sequence, a V-type I g domain (aa 36-115), one potential N-linked glycosylation site, and a single transmembrane domain. The V-type I g domain of mouse VSIG4 shares 86% and 8% aa sequence identity with the V-type I g domains of rat and human VSIG4, respectively.

References

Vogt, L. et al., 2006, J Clin Invest.116: 2817-2826. Helmy, K. et al., 2006, Cell. 124:915-927. Wiesmann, C. et al., 2006, Nature. 444:217-220. Zang, X. et al., 2006, J Clin Invest. 116: 2590-2593. Katschke, KJ. et al., 2007, J. Exp. Med. 204:1319-1325. He, JQ. et al., 2008, Mol Immunol. 45: 4041-4047.

NCBI and Uniprot Product Information

• NCBI GI #: 296434251
• NCBI GeneID: 11326
• NCBI Accession #: NP_001171759.1
• NCBI GenBank Nucleotide #: NM_001184830.1
• UniProt Accession #: Q9Y279; Q6UXI4
• Molecular Weight: 43,987 Da
• NCBI Official Full Name: V-set and immunoglobulin domain-containing protein 4 isoform 4
• NCBI Official Synonym Full Names: V-set and immunoglobulin domain containing 4
• NCBI Official Symbol: VSIG4
• NCBI Official Synonym Symbols: CRIg; Z39IG
• NCBI Protein Information: V-set and immunoglobulin domain-containing protein 4; protein Z39Ig; OTTHUMP00000023439; OTTHUMP00000023441; OTTHUMP00000023442; Ig superfamily protein; complement receptor of the immunoglobulin superfamily
• UniProt Protein Name: V-set and immunoglobulin domain-containing protein 4
• Protein Family: V-set and immunoglobulin domain-containing protein
• UniProt Gene Name: VSIG4
• UniProt Synonym Gene Names: CRIg; Z39IG
• UniProt Entry Name: VSIG4_HUMAN

NCBI Description

This gene encodes a v-set and immunoglobulin-domain containing protein that is structurally related to the B7 family of immune regulatory proteins. The encoded protein may be a negative regulator of T-cell responses. This protein is also a receptor for the complement component 3 fragments C3b and iC3b. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

VSIG4: Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: Xq12-q13.3

Cellular Component: integral to membrane

Molecular Function: protein binding

Biological Process: negative regulation of T cell proliferation; complement activation, alternative pathway; negative regulation of interleukin-2 production