Rabbit anti-Rat VRL-1 Polyclonal Antibody | anti-TRPV2 antibody
VRL-1 (Transient receptor potential cation channel subfamily V member 2, TrpV2, osm-9-like TRP channel 2, OTRPC2, Vanilloid receptor-like protein 1, Stretch-activated channel 2B)
Gene Names
TRPV2; VRL; VRL1; VRL-1; MGC12549; TRPV2
Reactivity
Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
VRL-1; Polyclonal Antibody; VRL-1 (Transient receptor potential cation channel subfamily V member 2; TrpV2; osm-9-like TRP channel 2; OTRPC2; Vanilloid receptor-like protein 1; Stretch-activated channel 2B); Anti -VRL-1 (Transient receptor potential cation channel subfamily V member 2; anti-TRPV2 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat Vanilloid receptor like protein 1 (VRL-1). No significant sequence homology is found with VR1 or other proteins. Conserved 100% in mouse VRL-1 and growth factor regulated calcium channel. Species sequence homology: mouse 100% and human 50%.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Applicable Applications for anti-TRPV2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1-10 ug/ml (ECL). Recombinant VR1 is ~90kD
ELISA: 0.5-1ug/ml. V2075-05A Control Peptide can be used to coat ELISA plates at 1ug/ml.
Immunohistochemistry: 1-20ug/ml.
Dilution: Western Blot: 1-10 ug/ml (ECL). Recombinant VR1 is ~90kD
ELISA: 0.5-1ug/ml. V2075-05A Control Peptide can be used to coat ELISA plates at 1ug/ml.
Immunohistochemistry: 1-20ug/ml.
Immunogen
Synthetic peptide (18aa) corresponding to the C-terminus of the rat VRL-1 protein.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-TRPV2 antibody
Capsaicin receptor, termed vanilloid receptor subtype 1 (VR1), has been cloned from sensory neurons. VR1 protein is a heat-gated cation channel. VR1 is also activated by noxious heat (elevation in temperature causing pain) and high proton concentrations (pH <6.0). Rat VR1 encodes a membrane protein of 838aa (~95kD) with 6 transmembrane domains. VR1 expression is limited to small to medium diameter primary sensory neurons. Recently, a structural homolog of VR1, termed VRL-1 (VR1 like proteins; rat 761aa, human 764aa) have been cloned and characterized. There is ~49% identity between VRL-1 and VR1. In contrast to VR1, VRL-1 does not respond to capsaicin, acid or moderate heat. Instead, it is activated by high temperatures with a threshold of ~52 degree C. Within sensory ganglia, VRL-1 is most prominently expressed by a subset of medium to large-diameter neurons, and in many non-neuronal tissues (lung, spleen and intestine) that are unlikely to encounter temperatures of >50 degree C. It is possible that VRL-1 is also activated by stimuli other than heat.
Product Categories/Family for anti-TRPV2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
85,981 Da
NCBI Official Full Name
vanilloid receptor-like protein 1
NCBI Official Synonym Full Names
transient receptor potential cation channel, subfamily V, member 2
NCBI Official Symbol
TRPV2
NCBI Official Synonym Symbols
VRL; VRL1; VRL-1; MGC12549; TRPV2
NCBI Protein Information
transient receptor potential cation channel subfamily V member 2; OTRPC2; osm-9-like TRP channel 2; vanilloid receptor-like protein 1
UniProt Protein Name
Transient receptor potential cation channel subfamily V member 2
Protein Family
UniProt Gene Name
TRPV2
UniProt Synonym Gene Names
VRL
UniProt Entry Name
TRPV2_HUMAN
NCBI Description
This gene encodes an ion channel that is activated by high temperatures above 52 degrees Celsius. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thought that in other tissues the channel may be activated by stimuli other than heat. [provided by RefSeq]
Uniprot Description
TRPV2: Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF-I, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH. Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV2 sub-subfamily.
Protein type: Channel, cation; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 17p11.2
Cellular Component: cell surface; integral to plasma membrane; plasma membrane; melanosome; endomembrane system
Molecular Function: calcium channel activity; ion transmembrane transporter activity; ion channel activity; cation channel activity
Biological Process: response to temperature stimulus; response to heat; transport; sensory perception; positive regulation of axon extension; transmembrane transport
Research Articles on TRPV2
1. TRPV2 negatively controls glioma cell survival and proliferation, as well as resistance to Fas-induced apoptotic cell death in an ERK-dependent manner.
2. Findings establish a role for TRPV2 in PCa progression to the aggressive castration-resistant stage, prompting evaluation of TRPV2 as a potential prognostic marker and therapeutic target in the setting of advanced PCa.
3. TRPV2 plays a role in human hepatocarcinogenesis and might be a prognostic marker of patients with hepatocellular carcinoma (HCC).
4. TRPV1-immunoreactivity was significantly higher and TRPV2-immunoreactivity was significantly lower in peripheral blood mononuclear cells from end-stage kidney disease patients compared to controls.
5. The reduction of TRPV2 and TRPM8 immunoreactive nerve fibers in skin from individuals with Norrbottnian congenital insensitivity to pain further suggests that these ion channels are expressed primarily on nociceptive primary sensory neurons.
6. A progressive loss of s-TRPV2 accompanied by an increase of hTRPV2 expression was found in high-grade and -stage UC.
7. relative gene expression ofTRPV1-4 in leukocytes is: TRPV3 < TRPV4, TRPV1 and TRPV2; leukocytes in hyposensitive subjects showed up-regulation of TRPV1, which was almost doubly expressed
8. Messenger RNA transcripts of TRPVs 1 through 4 are detected for the first time in human pulmonary artery smooth muscle cells.
9. It is concluded that PI3-kinase induces or modulates the activity of recombinant TRPV2 channels; in contrast to the previously proposed mechanism, activation of TRPV2 channels by PI3-kinase is not due to channel translocation to the plasma membrane.
10. TRPV2 and PKA have roles as a signaling module for transduction of physical stimuli in mast cells
2. Findings establish a role for TRPV2 in PCa progression to the aggressive castration-resistant stage, prompting evaluation of TRPV2 as a potential prognostic marker and therapeutic target in the setting of advanced PCa.
3. TRPV2 plays a role in human hepatocarcinogenesis and might be a prognostic marker of patients with hepatocellular carcinoma (HCC).
4. TRPV1-immunoreactivity was significantly higher and TRPV2-immunoreactivity was significantly lower in peripheral blood mononuclear cells from end-stage kidney disease patients compared to controls.
5. The reduction of TRPV2 and TRPM8 immunoreactive nerve fibers in skin from individuals with Norrbottnian congenital insensitivity to pain further suggests that these ion channels are expressed primarily on nociceptive primary sensory neurons.
6. A progressive loss of s-TRPV2 accompanied by an increase of hTRPV2 expression was found in high-grade and -stage UC.
7. relative gene expression ofTRPV1-4 in leukocytes is: TRPV3 < TRPV4, TRPV1 and TRPV2; leukocytes in hyposensitive subjects showed up-regulation of TRPV1, which was almost doubly expressed
8. Messenger RNA transcripts of TRPVs 1 through 4 are detected for the first time in human pulmonary artery smooth muscle cells.
9. It is concluded that PI3-kinase induces or modulates the activity of recombinant TRPV2 channels; in contrast to the previously proposed mechanism, activation of TRPV2 channels by PI3-kinase is not due to channel translocation to the plasma membrane.
10. TRPV2 and PKA have roles as a signaling module for transduction of physical stimuli in mast cells
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Product Notes
The TRPV2 trpv2 (Catalog #AAA610882) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VRL-1 (Transient receptor potential cation channel subfamily V member 2, TrpV2, osm-9-like TRP channel 2, OTRPC2, Vanilloid receptor-like protein 1, Stretch-activated channel 2B) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's VRL-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: Western Blot: 1-10 ug/ml (ECL). Recombinant VR1 is ~90kD ELISA: 0.5-1ug/ml. V2075-05A Control Peptide can be used to coat ELISA plates at 1ug/ml. Immunohistochemistry: 1-20ug/ml. Researchers should empirically determine the suitability of the TRPV2 trpv2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VRL-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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