Rabbit anti-Human VRK1 Polyclonal Antibody | anti-VRK1 antibody

Anti-VRK1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8105413

• Gene Names: VRK1; PCH1; PCH1A
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: VRK1; Polyclonal Antibody; Anti-VRK1 Antibody; Rabbit Polyclonal; VRK1 Antibody; Rabbit PAb; Antigen Affinity Purified; vaccinia related kinase 1; Anti-PCH1 Antibody; Anti-PCH1A Antibody; anti-VRK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human VRK1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-VRK1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human VRK1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human VRK1 (rh VRK1). VRK1 specific IgG was purified by Human VRK1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(VRK1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-VRK1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-VRK1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 45 kDaObserved band size: 45 kDa)

Western Blot (WB)

(Anti-VRK1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:45 kDaObserved band size:54 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-VRK1 antibody

VRK1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. Serine/threonine protein kinases are tumor suppressor that controls the activity of AMP-activated protein kinase family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. VRK1 contains 1 protein kinase domain and localizes to the nucleus. VRK1 gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. As a serine/threonine kinase, VRK1 phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1), also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy.

References

Sugimoto J, et al. (1999) Isolation and mapping of a polymorphic CA repeat sequence at the human VRK1 locus. J Hum Genet. 44 (2): 133-4.Lopez-Borges S, et al. (2000) The human vaccinia-related kinase 1 (VRK1) phosphorylates threonine-18 within the mdm-2 binding site of the p53 tumour suppressor protein. Oncogene. 19 (32): 3656-64.Sevilla A, et al. (2004) c-Jun phosphorylation by the human vaccinia-related kinase 1 (VRK1) and its cooperation with the N-terminal kinase of c-Jun (JNK). Oncogene. 23 (55): 8950-8.

NCBI and Uniprot Product Information

• NCBI GI #: 109730507
• NCBI GeneID: 7443
• UniProt Accession #: Q99986; Q3SYL2
• Molecular Weight: 45,476 Da
• NCBI Official Full Name: Vaccinia related kinase 1
• NCBI Official Synonym Full Names: VRK serine/threonine kinase 1
• NCBI Official Symbol: VRK1
• NCBI Official Synonym Symbols: PCH1; PCH1A
• NCBI Protein Information: serine/threonine-protein kinase VRK1
• UniProt Protein Name: Serine/threonine-protein kinase VRK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: VRK1

NCBI Description

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008]

Uniprot Description

Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.