Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Growth Differentiation Factor 1 (GDF1) Polyclonal Antibody | anti-GDF1 antibody
Polyclonal Antibody to Growth Differentiation Factor 1 (GDF1)
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Growth Differentiation Factor 1 (GDF1); Polyclonal Antibody; Polyclonal Antibody to Growth Differentiation Factor 1 (GDF1); anti-GDF1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GDF1. It has beenselected for its ability to recognize GDF1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-LGTAVAA NASVPRTLRL ALALHPGAAA TCGRLAEASL LLVTLDPRLC PLPRSRRHTE PRVGGGPVGT CRTRRLHVSF REVGWHRWVI APRGFLANFC QGTCALPETL RGPGGPPALN HAVLRALMHA AAPTPGVGSP CCVPERLSPI SVLFFDNSDN VVLRHYEDMV VDECGCR
MGHHHHHHSG SEF-LGTAVAA NASVPRTLRL ALALHPGAAA TCGRLAEASL LLVTLDPRLC PLPRSRRHTE PRVGGGPVGT CRTRRLHVSF REVGWHRWVI APRGFLANFC QGTCALPETL RGPGGPPALN HAVLRALMHA AAPTPGVGSP CCVPERLSPI SVLFFDNSDN VVLRHYEDMV VDECGCR
Applicable Applications for anti-GDF1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Parafin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GDF1 (Leu183~Arg357) expressed in E.coli.
Cross Reactivity
Rat
Conjugation
No Conjugate
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2063140
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Pancreas Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Pancreas Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,641 Da
NCBI Official Full Name
embryonic growth/differentiation factor 1
NCBI Official Synonym Full Names
growth differentiation factor 1
NCBI Official Symbol
Gdf1
NCBI Protein Information
embryonic growth/differentiation factor 1
UniProt Protein Name
Growth differentiation factor 1
Protein Family
UniProt Gene Name
Gdf1
NCBI Description
This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site that is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. The mouse ortholog of this gene is involved in the establishment of left-right asymmetry in early embryogenesis and in neural development in later embryogenesis. This protein is transcribed from a bicistronic mRNA that also encodes the LAG1 homolog, ceramide synthase 1 gene. Mono-cistronic transcripts for this gene are also found in mouse, but it is not known if they exist in rat. [provided by RefSeq, Jul 2009]
Research Articles on GDF1
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Product Notes
The GDF1 gdf1 (Catalog #AAA2004055) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Growth Differentiation Factor 1 (GDF1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Growth Differentiation Factor 1 (GDF1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Parafin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GDF1 gdf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-LGTAVA A NASVPRTLRL ALALHPGAAA TCGRLAEASL LLVTLDPRLC PLPRSRRHTE PRVGGGPVGT CRTRRLHVSF REVGWHRWVI APRGFLANFC QGTCALPETL RGPGGPPALN HAVLRALMHA AAPTPGVGSP CCVPERLSPI SVLFFDNSDN VVLRHYEDMV VDECGCR. It is sometimes possible for the material contained within the vial of "Growth Differentiation Factor 1 (GDF1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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