Rabbit anti-Mouse VPS54 Polyclonal Antibody | anti-VPS54 antibody

VPS54, ID (VPS54, HCC8, Vacuolar protein sorting-associated protein 54, Hepatocellular carcinoma protein 8, Tumor antigen HOM-HCC-8, Tumor antigen SLP-8p) (APC)

Cat. Num. AAA6362786

• Gene Names: VPS54; WR; HCC8; SLP-8p; VPS54L; hVps54L; PPP1R164
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: VPS54; Polyclonal Antibody; ID (VPS54; HCC8; Vacuolar protein sorting-associated protein 54; Hepatocellular carcinoma protein 8; Tumor antigen HOM-HCC-8; Tumor antigen SLP-8p) (APC); Tumor antigen SLP-8p; anti-VPS54 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-VPS54 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: VPS54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 464-491 amino acids from the Central region of human VPS54.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(VPS54 Antibody (Center) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the VPS54 antibody detected the VPS54 protein (arrow).)

Immunohistochemistry (IHC)

(VPS54 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VPS54 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-VPS54 antibody

This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms

Product Categories/Family for anti-VPS54 antibody

Antibodies; Cancer Markers

References

Perez-Victoria, F.J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(29):12860-12865(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Perez-Victoria, F.J., et al. Mol. Cell. Biol. 29(19):5251-5263(2009). Meisler, M.H., et al. Amyotroph Lateral Scler 9(3):141-148(2008). Perez-Victoria, F.J., et al. Mol. Biol. Cell 19(6):2350-2362(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 54234040
• NCBI GeneID: 51542
• NCBI Accession #: NP_001005739.1
• NCBI GenBank Nucleotide #: NM_001005739.1
• UniProt Accession #: Q9P1Q0; Q5VIR5; Q86YF7; Q8N6G3; Q9NPV0; Q9NT07; Q9NUJ0
• Molecular Weight: 15,982 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 54 isoform 2
• NCBI Official Synonym Full Names: vacuolar protein sorting 54 homolog (S. cerevisiae)
• NCBI Official Symbol: VPS54
• NCBI Official Synonym Symbols: WR; HCC8; SLP-8p; VPS54L; hVps54L; PPP1R164
• NCBI Protein Information: vacuolar protein sorting-associated protein 54; tumor antigen SLP-8p; tumor antigen HOM-HCC-8; hepatocellular carcinoma protein 8; protein phosphatase 1, regulatory subunit 164
• UniProt Protein Name: Vacuolar protein sorting-associated protein 54
• UniProt Gene Name: VPS54
• UniProt Synonym Gene Names: HCC8

NCBI Description

This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms [provided by RefSeq, Jul 2008]

Uniprot Description

Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD (PubMed:18367545). Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling (PubMed:25799061).