Rabbit anti-Human VPS4B Polyclonal Antibody | anti-VPS4B antibody

VPS4B, CT (VPS4B, SKD1, VPS42, Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K(+) transport growth defect 1) (PE)

Cat. Num. AAA6362773

• Gene Names: VPS4B; MIG1; SKD1; SKD1B; VPS4-2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: VPS4B; Polyclonal Antibody; CT (VPS4B; SKD1; VPS42; Vacuolar protein sorting-associated protein 4B; Cell migration-inducing gene 1 protein; Suppressor of K(+) transport growth defect 1) (PE); Suppressor of K(+) transport growth defect 1; anti-VPS4B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-VPS4B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: VPS4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-332 amino acids from the C-terminal region of human VPS4B.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(VPS4B Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the VPS4B antibody detected the VPS4B protein (arrow).)

Related Product Information for anti-VPS4B antibody

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq].

Product Categories/Family for anti-VPS4B antibody

Antibodies; Transport Proteins

References

Morita, E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(29):12889-12894(2010). McDonough, C.W., et al. Hum. Genet. (2009). In press: Bruce, E.A., et al. Virology 390(2):268-278(2009). Inoue, M., et al. Traffic 9(12):2180-2189(2008). Stuchell-Brereton, M.D., et al. Nature 449(7163):740-744(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 17865802
• NCBI GeneID: 9525
• NCBI Accession #: NP_004860.2
• NCBI GenBank Nucleotide #: NM_004869.3
• UniProt Accession #: O75351; Q69HW4; Q9GZS7
• Molecular Weight: 49,302 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 4B
• NCBI Official Synonym Full Names: vacuolar protein sorting 4 homolog B (S. cerevisiae)
• NCBI Official Symbol: VPS4B
• NCBI Official Synonym Symbols: MIG1; SKD1; SKD1B; VPS4-2
• NCBI Protein Information: vacuolar protein sorting-associated protein 4B; cell migration-inducing 1; cell migration-inducing gene 1 protein; suppressor of K(+) transport growth defect 1; suppressor of K+ transport defect 1; vacuolar protein sorting 4B
• UniProt Protein Name: Vacuolar protein sorting-associated protein 4B
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS4B
• UniProt Synonym Gene Names: SKD1; VPS42; Protein SKD1
• UniProt Entry Name: VPS4B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq, Jul 2008]

Uniprot Description

VPS4B: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Belongs to the AAA ATPase family.

Protein type: Vesicle; EC 3.6.4.6

Chromosomal Location of Human Ortholog: 18q21.33

Cellular Component: spindle pole; centrosome; lysosome; late endosome membrane; vacuolar membrane; early endosome; cytoplasm; late endosome; endosome membrane; nucleus; cytosol; endosome

Molecular Function: protein C-terminus binding; protein binding; ATPase activity; ATPase activity, coupled; microtubule-severing ATPase activity; ATP binding

Biological Process: ubiquitin-dependent protein catabolic process via the multivesicular body pathway; viral reproduction; endosome organization and biogenesis; cell separation during cytokinesis; intracellular cholesterol transport; regulation of viral reproduction; viral infectious cycle; endosome transport; response to lipid; protein depolymerization; mitotic metaphase plate congression; endosome to lysosome transport via multivesicular body sorting pathway; cytoplasmic microtubule organization and biogenesis; protein transport; potassium ion transport; nuclear organization and biogenesis; vacuole organization and biogenesis