Rabbit anti-Human TMPRSS9 Polyclonal Antibody | anti-TMPRSS9 antibody

TMPRSS9, NT (TMPRSS9, Transmembrane protease serine 9, Polyserase-I, Polyserine protease 1, Serase-1, Serase-2, Serase-3) (FITC)

Cat. Num. AAA6356771

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TMPRSS9; Polyclonal Antibody; NT (TMPRSS9; Transmembrane protease serine 9; Polyserase-I; Polyserine protease 1; Serase-1; Serase-2; Serase-3) (FITC); Serase-3; anti-TMPRSS9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TMPRSS9 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TMPRSS9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-153 amino acids from the N-terminal region of human TMPRSS9.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TMPRSS9 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TMPRSS9 antibody detected the TMPRSS9 protein (arrow).)

Related Product Information for anti-TMPRSS9 antibody

Serase-1 and serase-2 are serine proteases that hydrolyze the peptides N-t-Boc-Gln-Ala-Arg-AMC and N-t-Boc-Gln-Gly-Arg-AMC. In contrast, N-t-Boc-Ala-Phe-Lys-AMC and N-t-Boc-Ala-Pro-Ala-AMC are not significantly hydrolyzed.

Product Categories/Family for anti-TMPRSS9 antibody

Antibodies; Transmembrane Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Okumura, Y., et al. Biochem. J. 400(3):551-561(2006). Cal, S., et al. Proc. Natl. Acad. Sci. U.S.A. 100(16):9185-9190(2003). Cal, S., et al. Proc. Natl. Acad. Sci. U.S.A. 100(16):9185-9190(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 33667063
• NCBI GeneID: 360200
• NCBI Accession #: NP_892018.1
• NCBI GenBank Nucleotide #: NM_182973.1
• UniProt Accession #: Q7Z410; Q6ZND6; Q7Z411
• Molecular Weight: 114,021 Da
• NCBI Official Full Name: transmembrane protease serine 9
• NCBI Official Synonym Full Names: transmembrane protease, serine 9
• NCBI Official Symbol: TMPRSS9
• NCBI Protein Information: transmembrane protease serine 9; polyserase 1; polyserase-1; polyserase-I; polyserine protease 1; transmembrane serine protease 9
• UniProt Protein Name: Transmembrane protease serine 9
• Protein Family: Transmembrane protease serine
• UniProt Gene Name: TMPRSS9
• UniProt Synonym Gene Names: Polyserase-1
• UniProt Entry Name: TMPS9_HUMAN

Uniprot Description

Function: Serase-1 and serase-2 are serine proteases that hydrolyze the peptides N-t-Boc-Gln-Ala-Arg-AMC and N-t-Boc-Gln-Gly-Arg-AMC. In contrast, N-t-Boc-Ala-Phe-Lys-AMC and N-t-Boc-Ala-Pro-Ala-AMC are not significantly hydrolyzed.

Enzyme regulation: Inhibited by serine protease inhibitors PMSF and 4-(2-aminoethyl)benzenesulfonyl fluoride, but not by EDTA. Ref.1

Subcellular location: Cell membrane; Single-pass type II membrane protein Ref.1.

Tissue specificity: Expressed in fetal human tissues, such as kidney, liver, lung and brain, and in a variety of tumor cell lines. Weakly expressed in adult tissues including skeletal muscle, liver, placenta and heart. Ref.1

Domain: The serine protease 1 and 2 domains are catalytically active, whereas the serine protease 3 domain lacks the essential Ser residue of the catalytic triad at position 1009 and is predicted to be inactive.

Post-translational modification: Proteolytically cleaved to generate 3 independent serine protease chains. The cleaved chains may remain attached to the membrane thanks to disufide bonds. It is unclear whether cleavage always takes place. Ref.1

Sequence similarities: Belongs to the peptidase S1 family.Contains 1 LDL-receptor class A domain.Contains 3 peptidase S1 domains.

Sequence caution: The sequence BAD18439.1 differs from that shown. Reason: Frameshift at position 776. The sequence CAD35759.1 differs from that shown. Reason: Aberrant splicing.