Western Blot (WB)
(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human tonsil tissue lysate, Primary antibody: 163213(VPS36 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Rabbit anti-Human VPS36 Polyclonal Antibody | anti-VPS36 antibody
VPS36 Rabbit pAb
Gene Names
VPS36; EAP45; C13orf9; CGI-145
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
VPS36; Polyclonal Antibody; VPS36 Rabbit pAb; EAP45; C13orf9; CGI-145; anti-VPS36 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-VPS36 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
Immunogen
Synthetic peptide of human VPS36
Conjugation
Unconjugated
Molecular Function
Vacuolar sorting proteins (VPSs) are required for trafficking normal endocytic and biosynthetic proteins to the vacuole and also play an important role in the budding process of cells. The ESCRT-II (endosomal sorting complex required for transport II) complex, which is involved in endocytosis of ubiquinated membrane proteins, is formed by Eap45, EAP30, and VPS25. These vacuolar sorting proteins are also involved in a multiprotein complex with RNA ploymerase II elongation factor (ELL). Eap45 (ELL-associated protein of 45kDa), also known as vacuolar protein-sorting-associated protein 36, is a 386 amino acid protein containing a GLUE (GRAM-like ubiquitin-binding in Eap45) domain that mediates the binding to ubiquitin and phophoinosides. Since Eap45 colocalizes with ubiquinated proteins on late endosomes, it is likely that Eap45 plays a role in the endosomal sorting of ubiquniated cargo. There are two isoforms of Eap45 that exist as a result of alternative splicing events.
Modification
Unmodified
Cell Position
Cytoplasm, Endosome, Membrane, Nucleus
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human tonsil tissue lysate, Primary antibody: 163213(VPS36 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Western Blot (WB)
(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human tonsil tissue lysate, Primary antibody: 163213(VPS36 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 163213(VPS36 Antibody) at dilution 1/30. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 163213(VPS36 Antibody) at dilution 1/30. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 163213(VPS36 Antibody) at dilution 1/30. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 163213(VPS36 Antibody) at dilution 1/30. (Original magnification: ×200))
Product Categories/Family for anti-VPS36 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,958 Da
NCBI Official Full Name
vacuolar protein-sorting-associated protein 36 isoform 1
NCBI Official Synonym Full Names
vacuolar protein sorting 36 homolog (S. cerevisiae)
NCBI Official Symbol
VPS36
NCBI Official Synonym Symbols
EAP45; C13orf9; CGI-145
NCBI Protein Information
vacuolar protein-sorting-associated protein 36; ELL-associated protein of 45 kDa; ESCRT-II complex subunit VPS36
UniProt Protein Name
Vacuolar protein-sorting-associated protein 36
Protein Family
UniProt Gene Name
VPS36
UniProt Synonym Gene Names
C13orf9; EAP45
UniProt Entry Name
VPS36_HUMAN
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Product Notes
The VPS36 vps36 (Catalog #AAA4754984) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VPS36 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VPS36 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the VPS36 vps36 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VPS36, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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