Rabbit VPS34 Polyclonal Antibody | anti-VPS34 antibody

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508/S288c)(Baker's yeast) VPS34 Polyclonal Antibody

• Gene Names: VPS34; END12; PEP15; STT8; VPL7; VPS7; VPT29
• Reactivity: Saccharomyces cerevisiae (strain ATCC 204508/S288c)(Baker's yeast)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: VPS34; Polyclonal Antibody; Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508/S288c)(Baker's yeast) VPS34 Polyclonal Antibody; VPS34 Antibody; Phosphatidylinositol 3-kinase VPS34; PI3-kinase VPS34; PI3K VPS34; PtdIns-3-kinase VPS34; EC 2.7.1.137; Carboxypeptidase Y-deficient protein 15; Vacuolar protein sorting-associated protein 34; Vacuolar protein-targeting protein 29; VPS34 END12 PEP15 VPL7 VPT29 YLR240W L9672.10; anti-VPS34 antibody

• Host: Rabbit
• Reactivity: Saccharomyces cerevisiae (strain ATCC 204508/S288c)(Baker's yeast)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-VPS34 antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) VPS34 protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 6323269
• NCBI GeneID: 850941
• NCBI Accession #: NP_013341.1
• NCBI GenBank Nucleotide #: NM_001182127.1
• UniProt Accession #: P22543; D6VYN8
• Molecular Weight: 100,921 Da
• NCBI Official Full Name: phosphatidylinositol 3-kinase VPS34
• NCBI Official Symbol: VPS34
• NCBI Official Synonym Symbols: END12; PEP15; STT8; VPL7; VPS7; VPT29
• NCBI Protein Information: phosphatidylinositol 3-kinase VPS34
• UniProt Protein Name: Phosphatidylinositol 3-kinase VPS34
• Protein Family: Probable phosphatidylinositol 3-kinase
• UniProt Gene Name: VPS34
• UniProt Synonym Gene Names: END12; PEP15; VPL7; VPT29; PI3-kinase VPS34; PI3K VPS34; PtdIns-3-kinase VPS34

Uniprot Description

Phosphatidylinositol 3-kinase required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). Its activation by VPS15 may lead to the phosphorylation of phosphatidylinositol in the sorting compartment membrane. Finally, it might also be involved in ethanol tolerance and cell wall integrity.