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WB (Western Blot)
(Detection of human NAT10 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody AAA212661 (lot AAA212661-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse NAT10 Polyclonal Antibody | anti-NAT10 antibody
Rabbit anti-NAT10 Antibody, Affinity Purified
Gene Names
NAT10; ALP; NET43
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NAT10, Antibody; Rabbit anti-NAT10 Antibody, Affinity Purified; Kre33; RNA cytidine acetyltransferase; NET43; N-acetyltransferase-like protein; N-acetyltransferase 10 (GCN5-related); ALP; 18S rRNA cytosine acetyltransferase; N-acetyltransferase 10; anti-NAT10 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NAT10 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 675 and 725
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human NAT10 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody AAA212661 (lot AAA212661-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human NAT10 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody AAA212661 (lot AAA212661-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human NAT10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody AAA212661 (lot AAA212661-1) used for IP at 6 ug per reaction. NAT10 was also immunoprecipitated by rabbit anti-NAT10 antibody BL15900. For blotting immunoprecipitated NAT10, AAA212661 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human NAT10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody AAA212661 (lot AAA212661-1) used for IP at 6 ug per reaction. NAT10 was also immunoprecipitated by rabbit anti-NAT10 antibody BL15900. For blotting immunoprecipitated NAT10, AAA212661 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohiostchemistry)
(Detection of human NAT10 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NAT10 (Cat. No. AAA212661 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human NAT10 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NAT10 (Cat. No. AAA212661 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse NAT10 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-NAT10 (Cat. No. AAA212661 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse NAT10 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-NAT10 (Cat. No. AAA212661 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-NAT10 antibody
N-acetyltransferase 10 (NAT10) has protein acetyltransferase activity in vitro. NAT10 can acetylate both histones and microtubules [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H0A0].
Product Categories/Family for anti-NAT10 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
107,297 Da
NCBI Official Full Name
N-acetyltransferase 10 isoform a
NCBI Official Synonym Full Names
N-acetyltransferase 10 (GCN5-related)
NCBI Official Symbol
NAT10
NCBI Official Synonym Symbols
ALP; NET43
NCBI Protein Information
N-acetyltransferase 10; N-acetyltransferase-like protein
UniProt Protein Name
N-acetyltransferase 10
UniProt Gene Name
NAT10
UniProt Synonym Gene Names
ALP; KIAA1709
UniProt Entry Name
NAT10_HUMAN
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Product Notes
The NAT10 nat10 (Catalog #AAA212661) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NAT10 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NAT10 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NAT10 nat10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAT10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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