Western Blot (WB)
(Western blot analysis of extracts from 293, using LGALS3BP antibody. Lane 1 was treated with the blocking peptide.)
Rabbit anti-Human, Mouse LGALS3BP Polyclonal Antibody | anti-LGALS3BP antibody
LGALS3BP Antibody
Gene Names
LGALS3BP; 90K; M2BP; gp90; CyCAP; BTBD17B; MAC-2-BP; TANGO10B
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
LGALS3BP; Polyclonal Antibody; LGALS3BP Antibody; 90K; 90K MAC 2 BP; Basement membrane autoantigen p105; BTBD17B; Galectin 3 binding protein; Galectin-3-binding protein; L3 antigen; Lectin galactoside binding soluble 3 binding protein; Lectin galactoside-binding soluble 3-binding protein; LG3BP_HUMAN; M2BP; Mac 2 binding protein; Mac 2 BP; Mac-2 BP; Mac-2-binding protein; Mac2 BP; MAC2BP; Serum protein 90K; TANGO10B; Transport and golgi organization 10 homolog B; Tumor associated antigen 90K; Tumor-associated antigen 90K; anti-LGALS3BP antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
LGALS3BP antibody detects endogenous levels of LGALS3BP.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
585
Applicable Applications for anti-LGALS3BP antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human LGALS3BP.
Subcellular Location
Secreted. Secreted > Extracellular Space > Extracellular Matrix.
Tissue Specificity
Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (88%), Bovine (100%), Horse (88%), Sheep (100%), Rabbit (88%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from 293, using LGALS3BP antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from 293, using LGALS3BP antibody. Lane 1 was treated with the blocking peptide.)
Immunohistochemistry (IHC)
(MBS9603307 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603307 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of A549 cells, using LGALS3BP antibody.)
Western Blot (WB)
(Western blot analysis of extracts of A549 cells, using LGALS3BP antibody.)
Related Product Information for anti-LGALS3BP antibody
Function: Promotes integrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
Subunit Structure: Homodimers and homomultimers. The multimers form ring-like structures with a diameter of 30-40 nm. Binds LGALS1 and LGALS3. Binds ITGB1, COL4A1, COL5A1, COL6A1, FN1 and NID. Interacts with the gamma-tubulin ring complex (gamma-TuRC), composed of gamma-tubulin, TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6 (PubMed:29162697). The unglycosylated form interacts with PDE4DIP isoform 13/MMG8/SMYLE; this interaction may connect a pericentrosomal complex, made of AKAP9, CDK5RAP2, EB1/MAPRE1 and PDE4DIP, to the gamma-tubulin ring complex (gamma-TuRC) to promote microtubule assembly and acetylation (PubMed:29162697).
Subunit Structure: Homodimers and homomultimers. The multimers form ring-like structures with a diameter of 30-40 nm. Binds LGALS1 and LGALS3. Binds ITGB1, COL4A1, COL5A1, COL6A1, FN1 and NID. Interacts with the gamma-tubulin ring complex (gamma-TuRC), composed of gamma-tubulin, TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6 (PubMed:29162697). The unglycosylated form interacts with PDE4DIP isoform 13/MMG8/SMYLE; this interaction may connect a pericentrosomal complex, made of AKAP9, CDK5RAP2, EB1/MAPRE1 and PDE4DIP, to the gamma-tubulin ring complex (gamma-TuRC) to promote microtubule assembly and acetylation (PubMed:29162697).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 65-90 kDa
Predicted: 66 kDa
Predicted: 66 kDa
NCBI Official Full Name
galectin-3-binding protein
NCBI Official Synonym Full Names
galectin 3 binding protein
NCBI Official Symbol
LGALS3BP
NCBI Official Synonym Symbols
90K; M2BP; gp90; CyCAP; BTBD17B; MAC-2-BP; TANGO10B
NCBI Protein Information
galectin-3-binding protein
UniProt Protein Name
Galectin-3-binding protein
Protein Family
UniProt Gene Name
LGALS3BP
UniProt Synonym Gene Names
M2BP; MAC2BP; Mac-2 BP
NCBI Description
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Using fluorescence in situ hybridization the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1. [provided by RefSeq, Jul 2008]
Uniprot Description
Promotes integrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
Research Articles on LGALS3BP
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Product Notes
The LGALS3BP lgals3bp (Catalog #AAA9603307) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LGALS3BP Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LGALS3BP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the LGALS3BP lgals3bp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LGALS3BP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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