VPS29 recombinant protein

VPS29 1-182aa, Human, His tag, E Coli

Cat. Num. AAA204355

• Gene Names: VPS29; DC7; DC15; PEP11
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: VPS29; VPS29 1-182aa; Human; His tag; E Coli; Vacuolar protein sorting-associated protein; DC15; DC7; PEP11; VPS29 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMMLVLV LGDLHIPHRC NSLPAKFKKL LVPGKIQHIL CTGNLCTKES YDYLKTLAGD VHIVRGDFDE NLNYPEQKVV TVGQFKIGLI HGHQVIPWGD MASLALLQRQ FDVDILISGH THKFEAFEHE NKFYINPGSA TGAYNALETN IIPSFVLMDI QASTVVTYVY QLIGDDVKVE RIEYKKP
• Sequence Length: 182

• Applicable Applications for VPS29 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for VPS29 recombinant protein

Vacuolar protein sorting 29, also known as VPS29, belongs to a group of genes coding for vacuolar protein sorting (VPS) proteins that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. It is a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases, from the prevacuolar endosome back to the Golgi. Also, VPS29 may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Recombinant human VPS29 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for VPS29 recombinant protein

Signal Transduction

References

Verges M., et al. (2004) Nat. Cell Biol. 6:763-769; Damen E., et al. (2006) Biochem. J. 398:399-409

NCBI and Uniprot Product Information

• NCBI GI #: 7706441
• NCBI GeneID: 51699
• NCBI Accession #: NP_057310
• NCBI GenBank Nucleotide #: NM_016226.4
• UniProt Accession #: Q9UBQ0; Q502Y5; Q6FIF8; Q6IAH3; Q9H0W0; Q9NRP1; Q9NRU7
• Molecular Weight: 23.2 kDa (207aa) confirmed by MALDI-TOF
• NCBI Official Full Name: vacuolar protein sorting-associated protein 29 isoform 1
• NCBI Official Synonym Full Names: VPS29, retromer complex component
• NCBI Official Symbol: VPS29
• NCBI Official Synonym Symbols: DC7; DC15; PEP11
• NCBI Protein Information: vacuolar protein sorting-associated protein 29
• UniProt Protein Name: Vacuolar protein sorting-associated protein 29
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS29
• UniProt Synonym Gene Names: hVPS29

NCBI Description

This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms and representing non-protein coding transcripts have been found for this gene. [provided by RefSeq, Aug 2013]

Uniprot Description

Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (Probable). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with ANKRD27 (PubMed:24856514).