Western Blot (WB)
(Staining (0.1ug/ml) of Human Spleen lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat VPS29 Polyclonal Antibody | anti-VPS29 antibody
Goat anti-VPS29 (C Terminus) Antibody
Western Blot: Approx 20+21kDa bands observed in Human Spleen lysates (calculated MW of 20.5kDa according to NP_057310.1 and 20.9kDa according to NP_476528.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
(Staining (0.1ug/ml) of Human Spleen lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0.1ug/ml) of Human Spleen lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms and representing non-protein coding transcripts have been found for this gene. [provided by RefSeq, Aug 2013]
Uniprot Description
VPS29: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro). Belongs to the VPS29 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.3; Vesicle
Chromosomal Location of Human Ortholog: 12q24
Cellular Component: cytoplasm; cytosol; early endosome; endosome; endosome membrane; intracellular membrane-bound organelle; late endosome; retromer complex
Molecular Function: metal ion binding; protein binding; protein transporter activity
Biological Process: intracellular protein transport; retrograde transport, endosome to Golgi; Wnt receptor signaling pathway
Research Articles on VPS29
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Product Notes
The VPS29 vps29 (Catalog #AAA420259) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-VPS29 (C Terminus) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's VPS29 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 20+21kDa bands observed in Human Spleen lysates (calculated MW of 20.5kDa according to NP_057310.1 and 20.9kDa according to NP_476528.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the VPS29 vps29 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DDVKVERIEY KKP. It is sometimes possible for the material contained within the vial of "VPS29, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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