Testing Data
(Gel: 10%SDS-PAGE Lysate: 40ug K562 cell Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 20 seconds)
Rabbit anti-Human GAGE7 Polyclonal Antibody | anti-GAGE7 antibody
GAGE7 Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Synonyms
GAGE7; Polyclonal Antibody; GAGE7 Antibody; AL4; CT4.7; GAGE7B; GAGE-7B; anti-GAGE7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total GAGE7 protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Concentration
1.3 mg/ml (varies by lot)
Sequence Length
117
Applicable Applications for anti-GAGE7 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western blotting: 1:200-1:1000
Immunohistochemistry: 1:50-1:200
Immunohistochemistry: 1:50-1:200
Immunogen Type
Peptide
Immunogen Description
Synthetic peptide corresponding to residues near the C terminal of human G antigen 12I
Target Name
GAGE7
Preparation and Storage
Store at -20 degree C
Testing Data
(Gel: 10%SDS-PAGE Lysate: 40ug K562 cell Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 20 seconds)
Testing Data
(Gel: 10%SDS-PAGE Lysate: 40ug K562 cell Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 20 seconds)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/20.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/20.)
Related Product Information for anti-GAGE7 antibody
This gene belongs to a family of genes that are expressed in a variety of tumors but not in normal tissues, except for the testis. The sequences of the family members are highly related but differ by scattered nucleotide substitutions. The antigenic peptide YYWPRPRRY, which is also encoded by several other family members, is recognized by autologous cytolytic T lymphocytes.
Product Categories/Family for anti-GAGE7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,978 Da
NCBI Official Full Name
G antigen 12F
NCBI Official Synonym Full Names
G antigen 12F
NCBI Official Symbol
GAGE12F
NCBI Protein Information
G antigen 12F
UniProt Protein Name
G antigen 12I
Protein Family
UniProt Gene Name
GAGE12I
UniProt Synonym Gene Names
GAGE-12I
UniProt Entry Name
GG12I_HUMAN
Uniprot Description
GAGE12I: an anti-apoptotic member of a family of the GAGE family of proteins that are expressed in a variety of tumors but not in normal tissues, except for the testis. Transfection of GAGE-7/7B confers resistance to Fas induced apoptosis. The antigenic peptide YYWPRPRRY, which is also encoded by several other family members, is recognized by autologous cytolytic T lymphocytes.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: Xp11.23 not on reference assembly
Similar Products
Product Notes
The GAGE7 gage12i (Catalog #AAA9412788) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GAGE7 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GAGE7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:200-1:1000 Immunohistochemistry: 1:50-1:200. Researchers should empirically determine the suitability of the GAGE7 gage12i for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAGE7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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