Rabbit VPS28 Polyclonal Antibody | anti-VPS28 antibody

VPS28 Polyclonal Antibody

Cat. Num. AAA3009660

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: VPS28; Polyclonal Antibody; VPS28 Polyclonal Antibody; Vacuolar protein sorting-associated protein 28 homolog; anti-VPS28 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: VPS28 polyclonal antibody detects endogenous levels of VPS28 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 221

• Applicable Applications for anti-VPS28 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant protein of human VPS28
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cells, using VPS28 antibody.)

Related Product Information for anti-VPS28 antibody

This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

NCBI and Uniprot Product Information

• NCBI GI #: 7705885
• NCBI GeneID: 51160
• NCBI Accession #: NP_057292.1
• NCBI GenBank Nucleotide #: NP_057292.1
• UniProt Accession #: Q9UK41
• Molecular Weight: 25kDa
• NCBI Official Full Name: vacuolar protein sorting-associated protein 28 homolog isoform 1
• NCBI Official Synonym Full Names: VPS28 subunit of ESCRT-I
• NCBI Official Symbol: VPS28
• NCBI Protein Information: vacuolar protein sorting-associated protein 28 homolog
• UniProt Protein Name: Vacuolar protein sorting-associated protein 28 homolog
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS28
• UniProt Synonym Gene Names: H-Vps28
• UniProt Entry Name: VPS28_HUMAN

NCBI Description

This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

VPS28: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Belongs to the VPS28 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: late endosome membrane; cytoplasm; plasma membrane; endosome membrane; cytosol; endosome

Molecular Function: protein binding; protein complex binding

Biological Process: viral reproduction; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; viral protein processing; negative regulation of protein ubiquitination; viral infectious cycle; virus assembly; endosome transport; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway