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Western Blot (WB) (WB Suggested Anti-VPS28 AntibodyPositive Control: Lane 1: 20ug mouse brain extract Lane 2: 20ug mouse brain extractPrimary Antibody Dilution : 1:500Secondary Antibody : Anti rabbit-HRPSecondry Antibody Dilution : 1:5,000Submitted by: Scott Wilson, University of Alabama at Birmingham)

Rabbit VPS28 Polyclonal Antibody | anti-VPS28 antibody

VPS28 antibody - N-terminal region

Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
Western Blot
Purity
Affinity Purified
Synonyms
VPS28; Polyclonal Antibody; VPS28 antibody - N-terminal region; anti-VPS28 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: MFHGIPATPGIGAPGNKPELYEEVKLYKNAREREKYDNMAELFAVVKTMQ
Sequence Length
221
Applicable Applications for anti-VPS28 antibody
Western Blot (WB)
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human VPS28
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(WB Suggested Anti-VPS28 AntibodyPositive Control: Lane 1: 20ug mouse brain extract Lane 2: 20ug mouse brain extractPrimary Antibody Dilution : 1:500Secondary Antibody : Anti rabbit-HRPSecondry Antibody Dilution : 1:5,000Submitted by: Scott Wilson, University of Alabama at Birmingham)

Western Blot (WB) (WB Suggested Anti-VPS28 AntibodyPositive Control: Lane 1: 20ug mouse brain extract Lane 2: 20ug mouse brain extractPrimary Antibody Dilution : 1:500Secondary Antibody : Anti rabbit-HRPSecondry Antibody Dilution : 1:5,000Submitted by: Scott Wilson, University of Alabama at Birmingham)

Western Blot (WB)

(WB Suggested Anti-VPS28 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Liver)

Western Blot (WB) (WB Suggested Anti-VPS28 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Liver)
Related Product Information for anti-VPS28 antibody
This is a rabbit polyclonal antibody against VPS28. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) invol
Product Categories/Family for anti-VPS28 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25kDa
NCBI Official Full Name
vacuolar protein sorting-associated protein 28 homolog isoform 1
NCBI Official Synonym Full Names
VPS28 subunit of ESCRT-I
NCBI Official Symbol
VPS28
NCBI Protein Information
vacuolar protein sorting-associated protein 28 homolog
UniProt Protein Name
Vacuolar protein sorting-associated protein 28 homolog
UniProt Gene Name
VPS28
UniProt Synonym Gene Names
H-Vps28
UniProt Entry Name
VPS28_HUMAN

NCBI Description

This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

VPS28: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Belongs to the VPS28 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: late endosome membrane; cytoplasm; plasma membrane; endosome membrane; cytosol; endosome

Molecular Function: protein binding; protein complex binding

Biological Process: viral reproduction; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; viral protein processing; negative regulation of protein ubiquitination; viral infectious cycle; virus assembly; endosome transport; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway

Research Articles on VPS28

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Product Notes

The VPS28 vps28 (Catalog #AAA3213036) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VPS28 antibody - N-terminal region reacts with Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's VPS28 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the VPS28 vps28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ. It is sometimes possible for the material contained within the vial of "VPS28, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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