Rabbit anti-Human VITRN Polyclonal Antibody | anti-VITRN antibody

VITRN, CT (VIT, Vitrin) (Biotin)

Cat. Num. AAA6362611

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: VITRN; Polyclonal Antibody; CT (VIT; Vitrin) (Biotin); VIT; Vitrin; anti-VITRN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-VITRN antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: VITRN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 574-603 amino acids from the C-terminal region of human VITRN.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(VITRN Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the VITRN antibody detected the VITRN protein (arrow).)

Immunohistochemistry (IHC)

(VITRN antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VITRN antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(VITRN Antibody (C-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-VITRN antibody

Promotes matrix assembly and cell adhesiveness (By similarity).

Product Categories/Family for anti-VITRN antibody

Antibodies; Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). Tomarev, S.I., et al. Invest. Ophthalmol. Vis. Sci. 44(6):2588-2596(2003). Whittaker, C.A., et al. Mol. Biol. Cell 13(10):3369-3387(2002). Mayne, R., et al. Biochem. Soc. Trans. 27(6):832-835(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 295789109
• NCBI GeneID: 5212
• NCBI Accession #: NP_001171442.1
• NCBI GenBank Nucleotide #: NM_001177971.1
• UniProt Accession #: Q6UXI7; Q6P7T3; Q96DM8; Q96DT1; Q9UDN0; A1A526; A6NKI9; A8K7Y4; E9PF47
• Molecular Weight: 71,754 Da
• NCBI Official Full Name: vitrin isoform 4
• NCBI Official Synonym Full Names: vitrin
• NCBI Official Symbol: VIT
• NCBI Protein Information: vitrin
• UniProt Protein Name: Vitrin
• UniProt Gene Name: VIT
• UniProt Entry Name: VITRN_HUMAN

NCBI Description

This gene encodes an extracellular matrix (ECM) protein. The protein may be associated with cell adhesion and migration. High levels of expression of the protein in specific parts of the brain suggest its likely role in neural development. [provided by RefSeq, Jun 2016]