Rabbit VISA / MAVS Polyclonal Antibody | anti-VISA antibody

Rabbit Polyclonal to Human VISA / MAVS

Cat. Num. AAA241349

• Gene Names: MAVS; IPS1; VISA; IPS-1; CARDIF
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: VISA / MAVS; Polyclonal Antibody; Rabbit Polyclonal to Human VISA / MAVS; Anti-VISA / MAVS Antibody (Internal) IHC-plus; MAVS; CARDIF; CARD adaptor inducing IFN-beta; IPS-1; KIAA1271; IFN-B promoter stimulator 1; VISA; IPS1; Human VISA; anti-VISA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: 17 amino acid peptide from near the center of human VISA.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 540

• Applicable Applications for anti-VISA antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (0.5 - 2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human MAVS / VISA.
• Immunogen Type: Synthetic peptide
• Immunogen: VISA / MAVS antibody was raised against synthetic peptide from human MAVS / VISA.
• Antigen Modification: Internal
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-MAVS antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of VISA in Mouse Brain cells with VISA antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of VISA in rat brain tissue lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.)

Related Product Information for anti-VISA antibody

Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 (MIM 603029) or by the cytoplasmic RNA helicases MDA5 (MIM 606951) and RIGI (ROBO3; MIM 608630).

NCBI and Uniprot Product Information

• NCBI GI #: 83776598
• NCBI GeneID: 57506
• NCBI Accession #: NP_065797.2
• NCBI GenBank Nucleotide #: NM_020746.4
• UniProt Accession #: Q7Z434; Q2HWT5; Q3I0Y2; Q5T7I6; Q86VY7; Q9H1H3; Q9H4Y1; A8K6X0; B2BD33; B2BD34; F5H6C8; M1P2Z0
• Molecular Weight: 15,012 Da
• NCBI Official Full Name: mitochondrial antiviral-signaling protein isoform 1
• NCBI Official Synonym Full Names: mitochondrial antiviral signaling protein
• NCBI Official Symbol: MAVS
• NCBI Official Synonym Symbols: IPS1; VISA; IPS-1; CARDIF
• NCBI Protein Information: mitochondrial antiviral-signaling protein; IFN-B promoter stimulator 1; CARD adaptor inducing IFN-beta; virus-induced signaling adaptor; virus-induced-signaling adapter; CARD adapter inducing interferon beta; putative NF-kappa-B-activating protein 031N; interferon beta promoter stimulator protein 1
• UniProt Protein Name: Mitochondrial antiviral-signaling protein
• UniProt Gene Name: MAVS
• UniProt Synonym Gene Names: IPS1; KIAA1271; VISA; MAVS; Cardif; IPS-1; VISA
• UniProt Entry Name: MAVS_HUMAN

NCBI Description

This gene encodes an intermediary protein necessary in the virus-triggered beta interferon signaling pathways. It is required for activation of transcription factors which regulate expression of beta interferon and contributes to antiviral immunity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

MAVS: Required for innate immune defense against viruses. Acts downstream of DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis. Interacts with DDX58/RIG-I, IFIH1/MDA5, TRAF2 and TRAF6. May interact with IRF3, FADD, RIPK1, CHUK and IKBKB. Does not interact with TBK1. Interacts with and is cleaved by HCV and hepatitis GB virus B NS3/4A proteases. Interacts with and is cleaved by HHAV protein 3ABC. Interacts with NLRX1. Interaction with NLRX1 requires the CARD domain. Interacts with PSMA7. Interacts with TRAFD1. Interacts (via C-terminus) with PCBP2 in a complex containing MAVS/IPS1, PCBP2 and ITCH. Interacts with CYLD. Interacts with SRC. Interacts with DHX58/LGP2 and IKBKE. Present in T-cells, monocytes, epithelial cells and hepatocytes. Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: peroxisomal membrane; mitochondrial outer membrane; mitochondrion; mitochondrial membrane; integral to membrane

Molecular Function: signal transducer activity; protein binding; CARD domain binding; protein kinase binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of protein import into nucleus, translocation; viral reproduction; signal transduction; positive regulation of tumor necrosis factor production; positive regulation of interleukin-8 production; negative regulation of viral genome replication; defense response to bacterium; positive regulation of transcription factor import into nucleus; positive regulation of interferon-beta production; innate immune response; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of defense response to virus by host; negative regulation of interferon type I production; activation of innate immune response; defense response to virus; positive regulation of interferon-alpha production