Rabbit anti-Human VHR Polyclonal Antibody | anti-VHR antibody

VHR antibody

Cat. Num. AAA9409604

• Gene Names: DUSP3; VHR
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: VHR; Polyclonal Antibody; VHR antibody; anti-VHR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 185

• Applicable Applications for anti-VHR antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:3000; Immunohistochemistry: 1:50-1:500
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 150 of Human DUSP3
• Target Name: VHR
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE Primary antibody diluted at 1: 1000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded N87 xenograft, using DUSP3 antibody at 1: 500 dilution.)

Related Product Information for anti-VHR antibody

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq]

Product Categories/Family for anti-VHR antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4758208
• NCBI GeneID: 1845
• NCBI Accession #: NP_004081
• NCBI GenBank Nucleotide #: NM_004090.3
• Molecular Weight: 16,163 Da
• NCBI Official Full Name: dual specificity protein phosphatase 3
• NCBI Official Synonym Full Names: dual specificity phosphatase 3
• NCBI Official Symbol: DUSP3
• NCBI Official Synonym Symbols: VHR
• NCBI Protein Information: dual specificity protein phosphatase 3
• UniProt Protein Name: Dual specificity protein phosphatase 3
• UniProt Gene Name: DUSP3
• UniProt Synonym Gene Names: VHR; VHR
• UniProt Entry Name: DUS3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq, Jul 2008]

Uniprot Description

DUSP3: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP3 inactivates the mitogen-activated kinases Erk2 and Jnk and is phosphorylated by ZAP-70. Phosphorylation by ZAP-70 is required for DUSP3 to inhibit the Erk2-Elk-1 pathway.

Protein type: EC 3.1.3.16; Protein phosphatase, dual-specificity; EC 3.1.3.48; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytoplasm; cytosol; immunological synapse; nucleoplasm; nucleus

Molecular Function: MAP kinase phosphatase activity; protein kinase binding; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity

Biological Process: inactivation of MAPK activity; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of JNK cascade; negative regulation of MAPKKK cascade; negative regulation of T cell activation; negative regulation of T cell receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of mitotic cell cycle; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway