Western Blot (WB)
(ZCRB1 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the ZCRB1 antibody detected the ZCRB1 protein (arrow).)
Rabbit ZCRB1 Polyclonal Antibody | anti-ZCRB1 antibody
ZCRB1 Antibody (C-term)
Western Blot (WB)
(ZCRB1 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the ZCRB1 antibody detected the ZCRB1 protein (arrow).)
Western Blot (WB)
(ZCRB1 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the ZCRB1 antibody detected the ZCRB1 protein (arrow).)
Immunohistochemistry (IHC)
(ZCRB1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZCRB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ZCRB1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZCRB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(ZCRB1 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ZCRB1 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
U12-type spliceosome binds U12-type pre-mRNAs and recognizes the 5'
splice site and branch-point sequence. U11 and U12 snRNPs are
components of U12-type spliceosome and function as a molecular
bridge connecting both ends of the intron. The protein encoded by
this gene contains a RNA recognition motif. It was identified as
one of the protein components of U11/U12 snRNPs. This protein and
many other U11/U12 snRNP proteins are highly conserved in organisms
known to contain U12-type introns. These proteins have been shown
to be essential for cell viability, suggesting the key roles in
U12-type splicing.
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Will, C.L., et al. RNA 10(6):929-941(2004)
NCBI and Uniprot Product Information
NCBI Description
Pre-mRNA splicing is catalyzed by the spliceosome. U12-type spliceosome binds U12-type pre-mRNAs and recognizes the 5' splice site and branch-point sequence. U11 and U12 snRNPs are components of U12-type spliceosome and function as a molecular bridge connecting both ends of the intron. The protein encoded by this gene contains a RNA recognition motif. It was identified as one of the protein components of U11/U12 snRNPs. This protein and many other U11/U12 snRNP proteins are highly conserved in organisms known to contain U12-type introns. These proteins have been shown to be essential for cell viability, suggesting the key roles in U12-type splicing. [provided by RefSeq, Jul 2008]
Uniprot Description
ZCRB1: Up-regulated by morphine. Down-regulated at 30-36 degrees Celsius while it is up-regulated at 39 degrees Celsius. Component of the U11/U12 snRNPs that are part of the U12- type spliceosome.
Protein type: RNA-binding
Chromosomal Location of Human Ortholog: 12q12
Cellular Component: nucleoplasm; U12-dependent spliceosome
Molecular Function: zinc ion binding; nucleotide binding
Biological Process: RNA splicing; mRNA processing
Research Articles on ZCRB1
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Product Notes
The ZCRB1 zcrb1 (Catalog #AAA9205310) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 184-212. The ZCRB1 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Bovine, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's ZCRB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the ZCRB1 zcrb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZCRB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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