Rabbit anti-Human, Mouse VGLL2 Polyclonal Antibody | anti-VGLL2 antibody

VGLL2, NT (VGLL2, VITO1, Transcription cofactor vestigial-like protein 2, Protein VITO1) (APC)

Cat. Num. AAA6362544

• Gene Names: VGLL2; VGL2; VITO1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: VGLL2; Polyclonal Antibody; NT (VGLL2; VITO1; Transcription cofactor vestigial-like protein 2; Protein VITO1) (APC); Protein VITO1; anti-VGLL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-VGLL2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: VGLL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-81 amino acids from the N-terminal region of human VGLL2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(VGLL2 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the VGLL2 antibody detected the VGLL2 protein (arrow).)

Related Product Information for anti-VGLL2 antibody

May act as a specific coactivator for the mammalian TEFs. May play a role in the development of skeletal muscles.

Product Categories/Family for anti-VGLL2 antibody

Antibodies; Transcription Factors

References

Lamesch, P., et al. Genomics 89(3):307-315(2007). Gunther, S., et al. Nucleic Acids Res. 32(2):791-802(2004). Maeda, T., et al. J. Biol. Chem. 277(50):48889-48898(2002). Mielcarek, M., et al. Mech. Dev. 119 SUPPL 1, S269-S274 (2002): Mielcarek, M., et al. Gene Expr. Patterns 2 (3-4), 305-310 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 23592242
• NCBI GeneID: 245806
• NCBI Accession #: NP_703154.1
• NCBI GenBank Nucleotide #: NM_153453.1
• UniProt Accession #: Q8N8G2; Q8WWX1
• Molecular Weight: 33,426 Da
• NCBI Official Full Name: transcription cofactor vestigial-like protein 2 isoform 2
• NCBI Official Synonym Full Names: vestigial like 2 (Drosophila)
• NCBI Official Symbol: VGLL2
• NCBI Official Synonym Symbols: VGL2; VITO1
• NCBI Protein Information: transcription cofactor vestigial-like protein 2; Vestigial and Tondu related protein 1
• UniProt Protein Name: Transcription cofactor vestigial-like protein 2
• Protein Family: Transcription cofactor vestigial-like protein
• UniProt Gene Name: VGLL2
• UniProt Synonym Gene Names: VITO1; Vgl-2
• UniProt Entry Name: VGLL2_HUMAN

NCBI Description

This gene encodes a protein with a transcriptional enhancer factor 1 (TEF-1) interaction domain. The encoded protein may act as a co-factor of TEF-1 regulated gene expression during skeletal muscle development. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]

Uniprot Description

Function: May act as a specific coactivator for the mammalian TEFs. May play a role in the development of skeletal muscles.

Subunit structure: Interacts with TEFs. Binds to TEAD1/TEF1.

Subcellular location: Nucleus

By similarity.

Tissue specificity: Skeletal muscle. Ref.1

Sequence similarities: Belongs to the vestigial family.