Rabbit Troponin T1 Polyclonal Antibody | anti-TNNT1 antibody

Troponin T1 Polyclonal Antibody

Cat. Num. AAA3006501

• Gene Names: TNNT1; ANM; TNT; NEM5; STNT; TNTS
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Troponin T1; Polyclonal Antibody; Troponin T1 Polyclonal Antibody; Troponin T; slow skeletal muscle; TnTs; Slow skeletal muscle troponin T; sTnT; TNNT1; TNT; Troponin T-SS; anti-TNNT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Troponin T1 polyclonal antibody detects endogenous levels of Troponin T1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 251

• Applicable Applications for anti-TNNT1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human Troponin T1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Troponin T1 polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:sp2/0 whole cell lysateLane3:Rat skeletal muscel tissue lysate)

Related Product Information for anti-TNNT1 antibody

Actin is a highly conserved protein that is expressed in all eukaryotic cells and interacts with Myosin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between Actin and Myosin by binding to calcium. Troponin is made up of at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Structures of skeletal muscle Troponin are composed of Troponin C (the sensor), Troponin I (the regulator) and three Troponin T (the link to the muscle thin filament) proteins, one of which functions as a Tropomyosin-binding protein and is known as Troponin T-SS (Troponin T-Slow Skeletal). Defects in the gene encoding Troponin T-SS are the cause of nemaline myopathy type 5 (NEM5), a form of nemaline myopathy characterized by mild contractures of the shoulders and hips, tremors and respiratory problems that often lead to death. Troponin T-SS is expressed as three isoforms due to alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 187173292
• NCBI GeneID: 7138
• NCBI Accession #: NP_001119605.1
• NCBI GenBank Nucleotide #: NP_001119605.1
• UniProt Accession #: P13805
• Molecular Weight: ~ 23kDa
• NCBI Official Full Name: troponin T, slow skeletal muscle isoform c
• NCBI Official Synonym Full Names: troponin T1, slow skeletal type
• NCBI Official Symbol: TNNT1
• NCBI Official Synonym Symbols: ANM; TNT; NEM5; STNT; TNTS
• NCBI Protein Information: troponin T, slow skeletal muscle
• UniProt Protein Name: Troponin T, slow skeletal muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNT1
• UniProt Synonym Gene Names: TNT; TnTs; sTnT
• UniProt Entry Name: TNNT1_HUMAN

NCBI Description

This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TNNT1: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT1 are the cause of nemaline myopathy type 5 (NEM5); also known as Amish nemaline myopathy (ANM). A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 5 is a severe and progressive form common among Old Order Amish. Affected infants display tremors with hypotonia and mild contractures of the shoulders and hips. Proximal contractures progressively weaken and a pectus carinatum deformity develops before children die of respiratory insufficiency, usually in the second year. Belongs to the troponin T family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Contractile

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: troponin complex; cytosol

Molecular Function: troponin T binding; tropomyosin binding

Biological Process: skeletal muscle contraction; slow-twitch skeletal muscle fiber contraction; negative regulation of muscle contraction; muscle filament sliding

Disease: Nemaline Myopathy 5