Rabbit VGF Polyclonal Antibody | anti-VGF antibody

Rabbit Polyclonal (IgG) to Human VGF

Cat. Num. AAA244203

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: VGF; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human VGF; Anti-VGF Antibody (aa454-503) IHC-plus; Neurosecretory protein VGF; Human VGF; anti-VGF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: VGF Antibody detects endogenous levels of total VGF protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 615

• Applicable Applications for anti-VGF antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:40000), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human VGF (aa454-503).
• Immunogen: VGF antibody was raised against synthetic peptide from human VGF (aa454-503).
• Antigen Modification: aa454-503
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-VGF antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of extracts from RAW264.7 cells, using VGF Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-VGF antibody

VGF encodes a 70-kD polypeptide that shares similarities with the secretogranin/ chromogranin family and is found in the secretory granules of subsets of neurons and endocrine cells. Expression of VGF is both developmentally regulated and in regulated in different brain regions in response to different stimuli. VGF has been shown to play a role in energy homeostasis. Expression of VGF in the hypothalamus of rats and mice is induced by exercise and in response to fasting.

NCBI and Uniprot Product Information

• NCBI GI #: 17136078
• NCBI GeneID: 7425
• NCBI Accession #: NP_003369.2
• NCBI GenBank Nucleotide #: NM_003378.3
• UniProt Accession #: O15240; Q9UDW8
• Molecular Weight: 67,258 Da
• NCBI Official Full Name: neurosecretory protein VGF
• NCBI Official Synonym Full Names: VGF nerve growth factor inducible
• NCBI Official Symbol: VGF
• NCBI Protein Information: neurosecretory protein VGF; neuro-endocrine specific protein VGF
• UniProt Protein Name: Neurosecretory protein VGF
• Protein Family: Neurosecretory protein
• UniProt Gene Name: VGF
• UniProt Synonym Gene Names: NERP-1; NERP-2
• UniProt Entry Name: VGF_HUMAN

NCBI Description

This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

VGF: May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular space; transport vesicle; cytoplasmic vesicle

Molecular Function: neuropeptide hormone activity; growth factor activity

Biological Process: response to dietary excess; generation of precursor metabolites and energy; response to cAMP; ovarian follicle development; insulin secretion; sexual reproduction; defense response to bacterium; response to cold; glucose homeostasis; response to insulin stimulus