Rabbit anti-Human Neurosecretory Protein VGF Polyclonal Antibody | anti-VGF antibody

Neurosecretory Protein VGF, CT (VGF)

Cat. Num. AAA642635

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Neurosecretory Protein VGF; Polyclonal Antibody; CT (VGF); Anti -Neurosecretory Protein VGF; anti-VGF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VGF. Species sequence homology: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-VGF antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:40,000; Western Blot: 1:500-1:1000; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to human VGF.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of extracts from RAW264.7 cells, using VGF Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-VGF antibody

VGF encodes a 70kD polypeptide that shares similarities with the secretogranin/ chromogranin family and is found in the secretory granules of subsets of neurons and endocrine cells. Expression of VGF is both developmentally regulated and in regulated in different brain regions in response to different stimuli. VGF has been shown to play a role in energy homeostasis. Expression of VGF in the hypothalamus of rats and mice is induced by exercise and in response to fasting.

Product Categories/Family for anti-VGF antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 17136078
• NCBI GeneID: 7425
• NCBI Accession #: NP_003369.2
• NCBI GenBank Nucleotide #: NM_003378.3
• UniProt Accession #: O15240; Q9UDW8
• Molecular Weight: 67,258 Da
• NCBI Official Full Name: neurosecretory protein VGF
• NCBI Official Synonym Full Names: VGF nerve growth factor inducible
• NCBI Official Symbol: VGF
• NCBI Protein Information: neurosecretory protein VGF; neuro-endocrine specific protein VGF
• UniProt Protein Name: Neurosecretory protein VGF
• Protein Family: Neurosecretory protein
• UniProt Gene Name: VGF
• UniProt Synonym Gene Names: NERP-1; NERP-2
• UniProt Entry Name: VGF_HUMAN

NCBI Description

This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

VGF: May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular space; transport vesicle; cytoplasmic vesicle

Molecular Function: neuropeptide hormone activity; growth factor activity

Biological Process: response to dietary excess; generation of precursor metabolites and energy; response to cAMP; ovarian follicle development; insulin secretion; sexual reproduction; defense response to bacterium; response to cold; glucose homeostasis; response to insulin stimulus