Rabbit VEGFB Polyclonal Antibody | anti-VEGFB antibody

VEGFB Antibody

Cat. Num. AAA9408448

• Gene Names: VEGFB; VRF; VEGFL
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: VEGFB; Polyclonal Antibody; VEGFB Antibody; VEGFL; VRF; VascularendothelialgrowthfactorB; VEGF-relatedfactor; anti-VEGFB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total VEGFB protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 188

• Applicable Applications for anti-VEGFB antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human VEGFB.
• Target Name: VEGFB
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of mouse heart cell lines, using VEGFB antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human endometrial cancer using VEGFB antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human Neurilemmoma using VEGFB antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human spleen hematoma using VEGFB antibody at dilution of 1:200 (200x lens). Immunohistochemical analysis of paraffin-embedded human stomach cancer using VEGFB antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human epididymis using VEGFB antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded)

Related Product Information for anti-VEGFB antibody

Vascular endothelial growth factor (VEGF) is a highly specific mitogen for vascular endothelial cells. VEGF and its close relatives VEGF-B, -C and -D form a subfamily within PDGF family of growth factors, which belongs to the cysteine knot class of cytokines. Five VEGF isoforms of 121, 145, 165, 189 and 206 amino acids (VEGF121¨C206) are generated as a result of alternative splicing from a single VEGF gene (1).The various VEGF forms bind to three tyrosine-kinase receptors, VEGFR-1, VEGFR-2 and VEGFR-3 which are expressed almost exclusively in endothelial cells. VEGFR-2 is the main angiogenic signal transducer for VEGF, while VEGFR-3 is specific for VEGF-C and -D and is necessary and sufficient for lymphangiogenic signaling. However, upon proteolytic processing VEGF-C and -D gain the ability to also bind and activate VEGFR-2 (2). Guided by the binding properties of the ligands, the VEGFRs are able to form both homodimers and heterodimers. Receptor dimerization is accompanied by activation of receptor kinase activity leading to receptor autophosphorylation. Phosphorylated receptors recruit interacting proteins and induce downstream signaling (3). Recently, tumor therapies based on neutralizing anti-VEGF antibodies and small molecule tyrosine kinase inhibitors targeting VEGFRs have been developed. These new strategies for tumor treatment show the clinical relevance of inhibiting VEGF signal transduction pathways that are exaggerated in pathological angiogenesis (4).

Product Categories/Family for anti-VEGFB antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 344179103
• NCBI GeneID: 7423
• NCBI Accession #: NP_001230662.1
• NCBI GenBank Nucleotide #: NM_001243733.1
• UniProt Accession #: P49765; Q16528
• Molecular Weight: 21,261 Da
• NCBI Official Full Name: vascular endothelial growth factor B isoform VEGFB-167
• NCBI Official Synonym Full Names: vascular endothelial growth factor B
• NCBI Official Symbol: VEGFB
• NCBI Official Synonym Symbols: VRF; VEGFL
• NCBI Protein Information: vascular endothelial growth factor B
• UniProt Protein Name: Vascular endothelial growth factor B
• Protein Family: Vascular endothelial growth factor
• UniProt Gene Name: VEGFB
• UniProt Synonym Gene Names: VRF; VEGF-B; VRF
• UniProt Entry Name: VEGFB_HUMAN

NCBI Description

This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclear-encoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Sep 2011]

Uniprot Description

VEGFB: Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. Homodimer; disulfide-linked. Can also form heterodimer with VEGF. Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Cell cycle regulation; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular region; extracellular space; membrane

Molecular Function: chemoattractant activity; growth factor activity; heparin binding; protein binding; protein heterodimerization activity; protein homodimerization activity; vascular endothelial growth factor receptor 1 binding

Biological Process: angiogenesis; blood coagulation; cardiac muscle contraction; induction of positive chemotaxis; negative regulation of apoptosis; negative regulation of neuron apoptosis; platelet activation; platelet degranulation; positive chemotaxis; positive regulation of cell division; positive regulation of endothelial cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of vascular permeability; protein amino acid O-linked glycosylation; response to drug; vascular endothelial growth factor receptor signaling pathway